Characterisation of the Urinary Metabolic Profile of Liver Fluke-Associated Cholangiocarcinoma
| العنوان: | Characterisation of the Urinary Metabolic Profile of Liver Fluke-Associated Cholangiocarcinoma |
|---|---|
| المؤلفون: | Alsaleh, Munirah, Sithithaworn, Paiboon, Khuntikeo, Narong, Loilome, Watcharin, Yongvanit, Puangrat, Chamadol, Nittaya, Hughes, Thomas, O'Connor, Thomas, Andrews, Ross H., Holmes, Elaine, Taylor-Robinson, Simon D. |
| المصدر: | Journal of Clinical and Experimental Hepatology |
| بيانات النشر: | Elsevier, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | carnitine palmitoyltransferase 2, CPT2, cholangiocarcinoma screening and care program, CASCAP, ultra-performance liquid chromatography mass spectrometry, UPLC-MS, reversed-phase ultra-performance liquid-chromatography mass spectrometry, RP-UPLC-MS, cholangiocarcinoma, CCA, hypoxanthine phosphoribosyltransferase 1, HPRT1, bile duct cancer, primary biliary cholangitis, PBC, electrospray ionisation, ESI, hypoxanthine-guanine phosphoribosyltransferase, HPRT, periportal fibrosis, PPF, parasitic diseases, orthogonal projections to latent structures discriminant analysis, OPLS-DA, primary sclerosing cholangitis, PSC, variable importance in projection, VIP, Original Article, metabonomics, acetaminophen, APAP, carnitine palmitoyltransferase 1, CPT1, periductal fibrosis, PDF, carnitine/acylcarnitine translocase, CACT, principal component analysis, PCA, mass spectrometry, Opisthorchis viverrini, data-dependent acquisition, DDA |
| الوصف: | Background Human infection with Opisthorchis viverrini, a carcinogenic liver fluke inhabiting the biliary tree, is endemic in Southeast Asia. Chronic infection is associated with a fatal complication, cholangiocarcinoma (CCA), a late-presenting and aggressive malignancy. Currently, annual mortality rates from CCA mirror trends in incidence, due in part to limited availability of efficient prognostic and early diagnostic biomarkers. With ability to detect thousands of urinary metabolites using metabonomics, the urine metabolome holds great potential in providing an insight into system-level alterations in carcinogenesis and in identifying metabolic markers altered in response to disturbed homoeostasis. Methods Global molecular profiling using reversed-phase ultraperformance liquid chromatography mass spectrometry was utilised to acquire the urinary spectral profile of 137 Thai subjects (48 at high risk of infection, 41 with O. viverrini infection, 34 periportal fibrosis and 14 CCA) from Khon Kaen, Thailand. Results Multivariate statistical analysis identified perturbation in several molecular classes related to purine metabolism and lipid metabolism in the CCA urine metabolome. These markers mainly reflect changes in energy metabolism to support proliferation (increased fatty acid oxidation and purine recycling), DNA methylation and hepatic injury. Conclusions Several metabolites of biological interest were discovered from this proof-of-principle dataset. Augmenting these findings is essential to accelerate the development of urinary metabolic markers in CCA. |
| اللغة: | English |
| تدمد: | 2213-3453 0973-6883 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::715c58ec71b39ae0670e8f2d37b8e932Test http://europepmc.org/articles/PMC6926188Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........715c58ec71b39ae0670e8f2d37b8e932 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22133453 09736883 |
|---|