Vitamin D attenuates cerebral artery remodeling through VDR/AMPK/eNOS dimer phosphorylation pathway after subarachnoid hemorrhage in rats
| العنوان: | Vitamin D attenuates cerebral artery remodeling through VDR/AMPK/eNOS dimer phosphorylation pathway after subarachnoid hemorrhage in rats |
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| المؤلفون: | John H. Zhang, Wing Mann Ho, Gaiqing Wang, Budbazar Enkhjargal, Jay Malaguit, Jiping Tang, Weifeng Wan, Wu Jiang |
| المصدر: | Journal of Cerebral Blood Flow & Metabolism. 39:272-284 |
| بيانات النشر: | SAGE Publications, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, medicine.medical_specialty, Subarachnoid hemorrhage, Nitric Oxide Synthase Type III, Endothelium, Cerebral arteries, AMP-Activated Protein Kinases, Vascular Remodeling, Calcitriol receptor, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Enos, Internal medicine, medicine, Animals, Vasospasm, Intracranial, cardiovascular diseases, Osteopontin, Phosphorylation, Cholecalciferol, biology, business.industry, AMPK, Original Articles, Cerebral Arteries, Subarachnoid Hemorrhage, medicine.disease, biology.organism_classification, Rats, Up-Regulation, nervous system diseases, 030104 developmental biology, Endocrinology, medicine.anatomical_structure, Neurology, biology.protein, Receptors, Calcitriol, Artery Endothelium, Neurology (clinical), Protein Multimerization, Cardiology and Cardiovascular Medicine, business, 030217 neurology & neurosurgery |
| الوصف: | The role of vitamin D3 (VitD3) in the upregulation of osteopontin (OPN) and eNOS in the endothelium of cerebral arteries after subarachnoid hemorrhage (SAH) is investigated. The endovascular perforation SAH model in Sprague-Dawley rats ( n = 103) was used. The VitD3 pretreatment (30 ng/kg) increased endogenous OPN and eNOS expression in cerebral arteries compared with naïve rats ( n = 5 per group). Neurobehavioral scores were significantly improved in Pre-SAH+VitD3 group compared with the SAH group. The effects of VitD3 were attenuated by intracerebroventricular (i.c.v) injections of siRNA for the vitamin D receptor (VDR) and OPN in Pre-SAH+VitD3+VDR siRNA and Pre-SAH+VitD3+OPN siRNA rats, respectively ( n = 5 per group). The significant increase of VDR, OPN and decrease of C44 splicing in the cerebral arteries of Pre-SAH+VitD3 rats lead to an increase in basilar artery lumen. The increase in VDR expression led to an upregulation and phosphorylation of AMPK and eNOS, especially dimer form, in endothelium of cerebral artery. The results provide that VitD3 pretreatment attenuates cerebral artery remodeling and vasospasm through the upregulation of OPN and phosphorylation of AMPK (p-AMPK) and eNOS (p-eNOS) at Ser1177-Dimer in the cerebral arteries. Vitamin D may be a useful new preventive and therapeutic strategy against cerebral artery remodeling in stroke patients. |
| تدمد: | 1559-7016 0271-678X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::60620ab4584bd6fa76e5979af9944cbcTest https://doi.org/10.1177/0271678x17726287Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....60620ab4584bd6fa76e5979af9944cbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15597016 0271678X |
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