Percutaneous transluminal renal angioplasty attenuates poststenotic kidney mitochondrial damage in pigs with renal artery stenosis and metabolic syndrome
| العنوان: | Percutaneous transluminal renal angioplasty attenuates poststenotic kidney mitochondrial damage in pigs with renal artery stenosis and metabolic syndrome |
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| المؤلفون: | Xiangyang Zhu, Hui Tang, Christopher M. Ferguson, Kyra L. Jordan, Amir Lerman, Mohsen Afarideh, Stephen C. Textor, Rahele A. Farahani, Alfonso Eirin, Lilach O. Lerman, Ishran M. Saadiq |
| المصدر: | J Cell Physiol |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Mean arterial pressure, medicine.medical_specialty, Swine, Physiology, medicine.medical_treatment, Clinical Biochemistry, Urology, Renal function, Kidney, Renal Artery Obstruction, urologic and male genital diseases, Renal artery stenosis, Revascularization, Article, 03 medical and health sciences, 0302 clinical medicine, medicine.artery, Animals, Medicine, Renal artery, Metabolic Syndrome, Renal ischemia, urogenital system, business.industry, Angioplasty, Hemodynamics, Endothelial Cells, Cell Biology, medicine.disease, Fibrosis, Mitochondria, Oxidative Stress, 030104 developmental biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Renal blood flow, Hypertension, business |
| الوصف: | OBJECTIVE: Percutaneous transluminal renal angioplasty (PTRA) has been used to treat renovascular disease (RVD), a chronic condition characterized by renal ischemia and metabolic abnormalities. Mitochondrial injury has been implicated as a central pathogenic mechanism in RVD, but whether it can be reversed by PTRA remains uncertain. We hypothesized that PTRA attenuates mitochondrial damage, renal injury and dysfunction in pigs with coexisting renal artery stenosis (RAS) and metabolic syndrome (MetS). METHODS: Four groups of pigs (n=6 each) were studied after 16 weeks of diet-induced MetS and RAS (MetS+RAS), MetS+RAS treated 4 weeks earlier with PTRA, and Lean and MetS Sham controls. Single-kidney renal blood flow (RBF) and glomerular filtration rate (GFR) were assessed in-vivo with multi-detector-CT, and renal tubular mitochondrial structure and function and renal injury ex-vivo. RESULTS: PTRA successfully restored renal artery patency, but mean arterial pressure remained unchanged. Stenotic kidney RBF and GFR, that fell in MetS+RAS compared to MetS, rose after PTRA. PTRA attenuated MetS+RAS-induced mitochondrial structural abnormalities in tubular cells and peritubular capillary endothelial cells, decreased mitochondrial H(2)0(2) production, and increased renal cytochrome-c oxidase-IV activity and ATP production. PTRA also improved cortical microvascular and peritubular capillary density and ameliorated tubular injury and tubulointerstitial fibrosis in the post-stenotic kidney. Importantly, renal mitochondrial damage correlated with post-stenotic injury and dysfunction. CONCLUSION: Renal revascularization attenuated mitochondrial injury and improved renal hemodynamics and function in swine post-stenotic kidneys. This study suggests a novel mechanism by which PTRA might be relatively effective in ameliorating mitochondrial damage and improving renal function in coexisting MetS and RAS. |
| تدمد: | 1097-4652 0021-9541 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::899660eafbf93911163b5d27521ec312Test https://doi.org/10.1002/jcp.30146Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....899660eafbf93911163b5d27521ec312 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974652 00219541 |
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