TGF-?1 enhances ?ig-h3-mediated keratinocyte cell migration through the ?3?1 integrin and PI3K
| العنوان: | TGF-?1 enhances ?ig-h3-mediated keratinocyte cell migration through the ?3?1 integrin and PI3K |
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| المؤلفون: | In San Kim, Ha Won Jeong |
| المصدر: | Journal of Cellular Biochemistry. 92:770-780 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | biology, Chemistry, Integrin, Cell migration, Cell Biology, Adhesion, Biochemistry, CD49c, Collagen receptor, Cell biology, Extracellular matrix, medicine.anatomical_structure, medicine, biology.protein, Keratinocyte, Molecular Biology, Protein kinase B |
| الوصف: | betaig-h3 is an extracellular matrix (ECM) protein whose expression is highly induced by transforming growth factor beta1 (TGF-beta1). We previously demonstrated that betaig-h3 has two alpha3beta1 integrin-interacting motifs, which promote adhesion, migration, and proliferation of human keratinocytes. Both betaig-h3 and TGF-beta1 have been suggested to play important roles in the healing of skin wounds. In this study, we demonstrate that TGF-beta1 enhances keratinocyte adhesion and migration toward betaig-h3 through the alpha3beta1 integrin. TGF-beta1 did not increase the amount of the alpha3beta1 integrin on the cell surface, but rather increased its affinity for betaig-h3. LY294002, an inhibitor of PI3K, blocked the basal and TGF-beta1-enhanced cell migration but not adhesion to betaig-h3. A constitutively active mutant of PI3K stimulated cell migration but not adhesion to betaig-h3. The PI3K pathway is also not associated with the affinity of the alpha3beta1 integrin to betaig-h3. TGF-beta1 induced phosphorylation of AKT and FAK. Taken together, these data suggest that TGF-beta1 increases affinity of the alpha3beta1 integrin to betaig-h3, resulting in enhanced adhesion and migration of keratinocytes toward betaig-h3. TGF-beta1 also enhances migration through PI3K, but PI3K is not associated with either the binding affinity of the alpha3beta1 integrin or its adhesion to betaig-h3. |
| تدمد: | 1097-4644 0730-2312 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::2572fcbd7df3c99a17ef06e91f6edd75Test https://doi.org/10.1002/jcb.20110Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........2572fcbd7df3c99a17ef06e91f6edd75 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
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