Association of the glucocorticoid receptor alternatively-spliced transcript 1A with the presence of the high molecular weight membrane glucocorticoid receptor in mouse lymphoma cells
| العنوان: | Association of the glucocorticoid receptor alternatively-spliced transcript 1A with the presence of the high molecular weight membrane glucocorticoid receptor in mouse lymphoma cells |
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| المؤلفون: | Cheryl S. Watson, Fanghong Chen, Bahiru Gametchu |
| المصدر: | Journal of Cellular Biochemistry. 74:430-446 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Untranslated region, DNA, Complementary, Time Factors, Lymphoma, Transcription, Genetic, Molecular Sequence Data, Gene Expression, HL-60 Cells, Biology, Transfection, Biochemistry, Dexamethasone, Primer extension, Mice, Receptors, Glucocorticoid, Glucocorticoid receptor, Complementary DNA, Tumor Cells, Cultured, medicine, Animals, Humans, Coding region, Cloning, Molecular, 3' Untranslated Regions, Glucocorticoids, Molecular Biology, Mice, Inbred BALB C, Base Sequence, Models, Genetic, cDNA library, Alternative splicing, Cell Biology, Flow Cytometry, Molecular biology, Molecular Weight, Alternative Splicing, COS Cells, Glucocorticoid, medicine.drug |
| الوصف: | Using the combination of a cDNA library prepared from membrane glucocorticoid (mGR)-enriched S-49 cells and a mouse leukocyte genomic library, we have cloned a 7.3 kb full-length glucocorticoid receptor 1A cDNA. Primer extension, 58RACE, and long distance PCR identified the transcription start site as being located at 1026 bp from the ATG codon. The first 1,013 nucleotides (nts) of the full length sequence constitute 58 UTR sequence (exon 1), the next 2349 bp, the coding region, and the last 3,907 bp, the 38UTR. The entire 5'UTR sequence is unique to transcript 1A. The 38UTR sequence is ,88.5 % conserved with the rat 38UTR. Western blot analysis compared the molecular weight of in vitro translation products from the cloned 1A cDNA with partially purified cellular mGR. Both preparations contained the novel 150 KD and the 94 KD classical GR peptides, suggesting that transcript 1A encodes both receptor forms. Transfection of mGR-less and glucocorticoid lysis-resistant AtT-20 and HL-60 cells with full-length GR 1A cDNA imparted both mGR expression and glucocorticoid lysis-sensitivity to these cells. J. Cell. Biochem. 74:430-446, 1999. r 1999 Wiley-Liss, Inc. |
| تدمد: | 1097-4644 0730-2312 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c2ca0f0db1d9d91c44418f0485072f3Test https://doi.org/10.1002Test/(sici)1097-4644(19990901)74:3<430::aid-jcb11>3.0.co;2-5 |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....1c2ca0f0db1d9d91c44418f0485072f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10974644 07302312 |
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