A novel circRNA, circNUP98, a potential biomarker, acted as an oncogene via the miR‐567/ PRDX3 axis in renal cell carcinoma
| العنوان: | A novel circRNA, circNUP98, a potential biomarker, acted as an oncogene via the miR‐567/ PRDX3 axis in renal cell carcinoma |
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| المؤلفون: | Rui Yu, Yu Ren, Jie Yao |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, Peroxiredoxin III, Microarray, Carcinogenesis, Apoptosis, urologic and male genital diseases, medicine.disease_cause, Mice, 0302 clinical medicine, Renal cell carcinoma, RNA interference, Middle Aged, Prognosis, Kidney Neoplasms, female genital diseases and pregnancy complications, Up-Regulation, Gene Expression Regulation, Neoplastic, Blot, 030220 oncology & carcinogenesis, Molecular Medicine, Female, Original Article, STAT3 Transcription Factor, renal cell carcinoma, circNUP98, Down-Regulation, Mice, Nude, Biology, Cell Line, PRDX3, 03 medical and health sciences, Circular RNA, Biomarkers, Tumor, medicine, Animals, Humans, Gene silencing, Carcinoma, Renal Cell, neoplasms, Oncogene, Oncogenes, RNA, Circular, Original Articles, Cell Biology, medicine.disease, Xenograft Model Antitumor Assays, miR‐567, MicroRNAs, HEK293 Cells, 030104 developmental biology, Cancer research |
| الوصف: | In recent years, plenty of studies found that circular RNAs (circRNAs) were essential players in the initiation and progression of various cancers including the renal cell carcinoma (RCC). However, the knowledge about the circRNAs in carcinogenesis is still limited. Dysregulated expression of circNUP98 in RCC tissues was identified by the circular RNA microarray. RT‐PCR was performed to measure the expression of circNUP98 in 78 pairs of RCC tissues and adjacent normal tissues. Survival analysis was conducted to explore the association between the expression of circNUP98 and the prognosis of RCC. The function and underlying mechanisms of circSMC3 in RCC cells were investigated by RNAi, CCK‐8, Western blotting, bioinformatic analysis, ChIP assay, circRIP assay and dual luciferase reporter assay. CircNUP98 was up‐regulated in both RCC tissues and cell lines, and high expression of circNUP98 was correlated with poor prognosis of RCC patients. Silencing of circSMC3 inhibited the proliferation and promoted the apoptosis in a caspase‐dependent manner in RCC cells. Mechanistically, we revealed that silencing of circ NUP98 inhibited RCC progression by down‐regulating of PRDX3 via up‐regulation of miR‐567. Furthermore, STAT3 was identified as an inducer of circ NUP98 in RCC cells. CircNUP98 acts as an oncogene by a novel STAT3/circ NUP98/miR‐567/PRDX3 axis, which may provide a potential biomarker and therapeutic target for the treatment of RCC. |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb36cbc4af23bb570c009a3291a76f93Test https://doi.org/10.1111/jcmm.15629Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fb36cbc4af23bb570c009a3291a76f93 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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