المؤلفون: Baorong He, Biao Wang, Lingbo Kong, Dingjun Hao, Liang Yan, Xiaobin Yang

المصدر: Journal of Cellular and Molecular Medicine

مصطلحات موضوعية: 0301 basic medicine, Integrins, Podosome, integrin, Integrin, Reviews, Osteoclasts, Review, Bone resorption, Mice, Phosphatidylinositol 3-Kinases, 03 medical and health sciences, 0302 clinical medicine, Osteogenesis, Osteoclast, Organelle, medicine, Animals, Humans, Syk Kinase, Bone Resorption, Cytoskeleton, Actin, biology, podosomes, Chemistry, Macrophage Colony-Stimulating Factor, RANK Ligand, cytoskeleton, Cell Biology, Cell biology, 030104 developmental biology, medicine.anatomical_structure, RANKL, 030220 oncology & carcinogenesis, osteoclast, biology.protein, Molecular Medicine, Signal Transduction

الوصف: In the ageing skeleton, the balance of bone reconstruction could commonly be broken by the increasing of bone resorption and decreasing of bone formation. Consequently, the bone resorption gradually occupies a dominant status. During this imbalance process, osteoclast is unique cell linage act the bone resorptive biological activity, which is a highly differentiated ultimate cell derived from monocyte/macrophage. The erosive function of osteoclasts is that they have to adhere the bone matrix and migrate along it, in which adhesive cytoskeleton recombination of osteoclast is essential. In that, the podosome is a membrane binding microdomain organelle, based on dynamic actin, which forms a cytoskeleton superstructure connected with the plasma membrane. Otherwise, as the main adhesive protein, integrin regulates the formation of podosome and cytoskeleton, which collaborates with the various molecules including: c‐Cbl, p130Cas, c‐Src and Pyk2, through several signalling cascades cross talking, including: M‐CSF and RANKL. In our current study, we discuss the role of integrin and associated molecules in osteoclastogenesis cytoskeletal, especially podosomes, regulation and relevant signalling cascades cross talking.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3f3d6d605d15fdc81e30b0f3cf4517cTest
https://doi.org/10.1111/jcmm.15052Test

المؤلفون: Wanli W. Smith, Rui Ma, Liang Yan, Yuan Liu, Lingbo Kong, Yang Cao, Xiaobin Yang

المصدر: Journal of Cellular and Molecular Medicine

مصطلحات موضوعية: 0301 basic medicine, musculoskeletal diseases, Cell, Osteoclasts, Cell lineage, 03 medical and health sciences, Mice, 0302 clinical medicine, Osteoclast, medicine, Animals, RAW264.7CRL‐2278, protocol, Cytoskeleton, Cell Proliferation, biology, RANK Ligand, RANKL, hemic and immune systems, Cell Differentiation, Cell Biology, Original Articles, In vitro, Cell biology, carbohydrates (lipids), 030104 developmental biology, medicine.anatomical_structure, RAW 264.7 Cells, 030220 oncology & carcinogenesis, osteoclast, biology.protein, Molecular Medicine, lipids (amino acids, peptides, and proteins), Original Article, RAW264.7TIB‐71, Cellular model, biological phenomena, cell phenomena, and immunity, Cell bank

الوصف: Although document studies (including ours) have been reported the achieved in vitro osteoclastic cellular model establishment from the RAW264.7 cell lineage, there was no study directly reported that American Type Culture Collection (ATCC) cell bank has various RAW264.7 cell lineages. Besides that, for our knowledge there was only one study compared the two different RAW264.7TIB‐71 and RAW264.7CRL‐2278 cell lineages for their osteoclastic differentiation, and they concluded that the RAW264.7CRL‐2278 demonstrated to generate much osteoclast than RAW264.7TIB‐71. However, on the contrary to their results we noticed the fusion of RAW264.7TIB‐71 in our previous studies was much compromising. Therefore, we try to explore the two cell lineages for their properties in osteoclastic differentiation with an in‐depth cellular cytoskeletal study. Our current study has showed that comparing to the RAW264.7CRL‐2278, RAW264.7TIB‐71 demonstrated a much higher efficacies for RANKL‐stimulated osteoclastic differentiation. Besides that, in our depth cytoskeletal studies, we found that the RANKL‐induced RAW264.7TIB‐71 cells could finally differentiate into mature osteoclasts. However, regardless the various pre‐treatment conditions, there was no mature osteoclast formed in RANKL‐induced RAW264.7CRL‐2278 cell lineage.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ee43907217e0baf00cbe24598f0437e8Test
https://pubmed.ncbi.nlm.nih.gov/33742541Test