التفاصيل البيبلوغرافية
| العنوان: |
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. |
| المؤلفون: |
Molli, Poonam R.1, Li, Da-Qiang1, Bagheri-Yarmand, Rozita2,3, Pakala, Suresh B.1, Katayama, Hiroshi4, Sen, Subrata4, Iyer, Jyoti5, Chernoff, Jonathan6, Tsai, Ming-Ying5, Nair, Sujit S.1, Kumar, Rakesh1,2 bcmrxk@gwumc.edu, Molli, P. R., Li, D.-Q |
| المصدر: |
Journal of Cell Biology. 7/12/2010, Vol. 190 Issue 1, p101-114. 14p. |
| مصطلحات موضوعية: |
*PROTEINS, *MITOSIS, *CENTROSOMES, *PHOSPHORYLATION, *GENETIC mutation |
| مستخلص: |
Here we provide evidence in support of an inherent role for Arpc1b, a component of the Arp2/3 complex, in regulation of mitosis and demonstrate that its depletion inhibits Aurora A activation at the centrosome and impairs the ability of mammalian cells to enter mitosis. We discovered that Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on threonine 21, and expression of Arpc1b but not a nonphosphorylatable Arpc1b mutant in mammalian cells leads to Aurora A kinase activation and abnormal centrosome amplification in a Pak1-independent manner. Together, these findings reveal a new function for Arpc1b in centrosomal homeostasis. Arpc1b is both a physiological activator and substrate of Aurora A kinase and these interactions help to maintain mitotic integrity in mammalian cells. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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