Suppression of KIF2 in PC12 Cells Alters the Distribution of a Growth Cone Nonsynaptic Membrane Receptor and Inhibits Neurite Extension
| العنوان: | Suppression of KIF2 in PC12 Cells Alters the Distribution of a Growth Cone Nonsynaptic Membrane Receptor and Inhibits Neurite Extension |
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| المؤلفون: | Alberto Rosa, Alfredo Cáceres, Kenneth S. Kosik, Gerardo Morfini, Santiago Quiroga |
| المصدر: | The Journal of Cell Biology |
| بيانات النشر: | Rockefeller University Press, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Neurite, medicine.medical_treatment, Synaptophysin, Kinesins, Nerve Tissue Proteins, Biology, PC12 Cells, Synaptic vesicle, Article, Receptor, IGF Type 1, Amyloid beta-Protein Precursor, GAP-43 Protein, Neurites, medicine, Amyloid precursor protein, Animals, Nerve Growth Factors, Gap-43 protein, Growth cone, Cerebral Cortex, Neurons, Organelles, Membrane Glycoproteins, Growth cone membrane, Growth factor, Antibodies, Monoclonal, Cell Biology, Oligonucleotides, Antisense, Thionucleotides, Synapsins, Rats, Up-Regulation, Cell biology, biology.protein, Synaptic Vesicles, Subcellular Fractions |
| الوصف: | In the present study, we present evidence about the cellular functions of KIF2, a kinesin-like superfamily member having a unique structure in that its motor domain is localized at the center of the molecule (Noda Y., Y. Sato-Yoshitake, S. Kondo, M. Nangaku, and N. Hirokawa. 1995. J. Cell Biol. 129:157–167.). Using subcellular fractionation techniques, isopicnic sucrose density centrifugation of microsomal fractions from developing rat cerebral cortex, and immunoisolation with KIF2 antibodies, we have now identified a type of nonsynaptic vesicle that associates with KIF2. This type of organelle lacks synaptic vesicle markers (synapsin, synaptophysin), amyloid precursor protein, GAP-43, or N-cadherin. On the other hand, it contains βgc, which is a novel variant of the β subunit of the IGF-1 receptor, which is highly enriched in growth cone membranes. Both βgc and KIF2 are upregulated by NGF in PC12 cells and highly concentrated in growth cones of developing neurons. We have also analyzed the consequences of KIF2 suppression by antisense oligonucleotide treatment on nerve cell morphogenesis and the distribution of synaptic and nonsynaptic vesicle markers. KIF2 suppression results in a dramatic accumulation of βgc within the cell body and in its complete disappearance from growth cones; no alterations in the distribution of synapsin, synaptophysin, GAP-43, or amyloid percursor protein are detected in KIF2-suppressed neurons. Instead, all of them remained highly enriched at nerve terminals. KIF2 suppression also produces a dramatic inhibition of neurite outgrowth; this phenomenon occurs after βgc has disappeared from growth cones. Taken collectively, our results suggest an important role for KIF2 in neurite extension, a phenomenon that may be related with the anterograde transport of a type of nonsynaptic vesicle that contains as one of its components a growth cone membrane receptor for IGF-1, a growth factor implicated in nerve cell development. |
| تدمد: | 1540-8140 0021-9525 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c6261453fac62ff0e8c02dc83b8eabdTest https://doi.org/10.1083/jcb.138.3.657Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3c6261453fac62ff0e8c02dc83b8eabd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15408140 00219525 |
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