The efficacy of EGFR gene mutation testing in various samples from non-small cell lung cancer patients: a multicenter retrospective study
| العنوان: | The efficacy of EGFR gene mutation testing in various samples from non-small cell lung cancer patients: a multicenter retrospective study |
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| المؤلفون: | Juliusz Pankowski, Monika Pasieka-Lis, Ewa Kalinka-Warzocha, Dariusz M. Kowalski, Janusz Milanowski, Aldona Woźniak, Jacek Jassem, Bożena Jarosz, Joanna Chorostowska-Wynimko, Bartosz Wasąg, Rodryg Ramlau, Marzena Anna Lewandowska, Maciej Krzakowski, Tomasz Powrózek, Justyna Szumiło, Paweł Krawczyk, Rafal Dziadziuszko, Kamila Wojas-Krawczyk, Wojciech Dyszkiewicz, Michal Skronski, Jerzy Kozielski, Ewelina Antoszewska, Janusz Limon, Wojciech Jóźwicki, Aleksandra Szczesna, Paulina Jaguś |
| المصدر: | Journal of Cancer Research and Clinical Oncology |
| بيانات النشر: | Springer Berlin Heidelberg, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Oncology, Male, Cancer Research, medicine.medical_specialty, Lung Neoplasms, DNA Mutational Analysis, Mutation testing, Gene mutation, Adenocarcinoma, EGFR Gene Mutation, TTF1, Polymerase Chain Reaction, Carcinoma, Adenosquamous, Non-small cell lung cancer, Internal medicine, Carcinoma, Non-Small-Cell Lung, medicine, Humans, Epidermal growth factor receptor, Lung cancer, Aged, Neoplasm Staging, Retrospective Studies, Tyrosine kinase inhibitors, Hematology, biology, Retrospective cohort study, General Medicine, Middle Aged, medicine.disease, Prognosis, DNA-Binding Proteins, ErbB Receptors, EGFR gene, Mutation, Cancer research, biology.protein, Carcinoma, Squamous Cell, Carcinoma, Large Cell, Female, Original Article – Cancer Research, Follow-Up Studies, Transcription Factors |
| الوصف: | Introduction Testing for the epidermal growth factor receptor (EGFR) gene mutations requires considerable multidisciplinary experience of clinicians (for appropriate patient selection), pathologists (for selection of appropriate cytological or histological material) and geneticists (for performing and reporting reliable molecular tests). We present our experience on the efficacy of routine EGFR testing in various types of tumor samples and the frequency of EGFR mutations in a large series of Polish non-small cell lung cancer (NSCLC) patients. Methods Deletions in exon 19 and substitution L858R in exon 21 of EGFR gene were assessed using real-time PCR techniques in 1,138 small biopsies or cytological specimens and in 1,312 surgical samples. Results Out of 2,450 diagnostic samples (containing >10 % of tumor cells), the occurrence of EGFR gene mutations was 9 %; more frequently in women (13.9 %) and adenocarcinoma patients (10 %), particularly with accompanying expression of TTF1 (13.0 %). The frequency of EGFR gene mutations was similar in cytological and histological specimens, and in primary and metastatic lesions, and did not depend on the percentage of tumor cells and quality of isolated DNA. Cytological or small biopsy, compared to surgical specimens showed lower percentage of tumor cells, with no impact on the quality of real-time PCR assay. Conclusion Cytological and small biopsy samples with low (10–20 %) content of tumor cells and specimens from metastatic lesions are a sufficient source for EGFR mutation testing in NSCLC patients. The incidence of EGFR gene mutations in examined population was similar to those reported in other Caucasian populations. |
| اللغة: | English |
| تدمد: | 1432-1335 0171-5216 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f47add329c687001afe9e2f5946dfdcbTest http://europepmc.org/articles/PMC4282872Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f47add329c687001afe9e2f5946dfdcb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14321335 01715216 |
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