Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell

التفاصيل البيبلوغرافية
العنوان: Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell
المؤلفون: Jing Lin, Hong Xu, Jingfei Zhu, Xianghui Qi, Yilin Qi, Qi Guo, Junhua Yun
المصدر: Journal of bioscience and bioengineering. 122(3)
سنة النشر: 2015
مصطلحات موضوعية: 0106 biological sciences, 0301 basic medicine, Gluconobacter oxydans, Bioengineering, Dehydrogenase, Reductase, Biology, medicine.disease_cause, Xylitol, 01 natural sciences, Applied Microbiology and Biotechnology, Metabolic engineering, 03 medical and health sciences, chemistry.chemical_compound, Xylulose, Sugar Alcohols, 010608 biotechnology, medicine, Escherichia coli, Cloning, Molecular, D-xylulose reductase, food and beverages, D-Xylulose Reductase, NAD, carbohydrates (lipids), Kinetics, 030104 developmental biology, chemistry, Biochemistry, Metabolic Engineering, Biotechnology
الوصف: Xylitol has numerous applications in food and pharmaceutical industry, and it can be biosynthesized by microorganisms. In the present study, xdh gene, encoding xylitol dehydrogenase (XDH), was cloned from the genome of Gluconobacter oxydans CGMCC 1.49 and overexpressed in Escherichia coli BL21. Sequence analysis revealed that XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/reductase family. And then, the enzymatic properties and kinetic parameter of purified recombinant XDH were investigated. Subsequently, transformations of xylitol from d-xylulose and d-arabitol, respectively, were studied through mixed culture of resting cells of G. oxydans wild-type strain and recombinant strain BL21-xdh. We obtained 28.80 g/L xylitol by mixed culture from 30 g/L d-xylulose in 28 h. The production was increased by more than three times as compared with that of wild-type strain. Furthermore, 25.10 g/L xylitol was produced by the mixed culture from 30 g/L d-arabitol in 30 h with a yield of 0.837 g/g, and the max volumetric productivity of 0.990 g/L h was obtained at 22 h. These contrast to the fact that wild-type strain G. oxydans only produced 8.10 g/L xylitol in 30 h with a yield of 0.270 g/g. To our knowledge, these values are the highest among the reported yields and productivity efficiencies of xylitol from d-arabitol with engineering strains.
تدمد: 1347-4421
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e7c9d737a2a640a9b5337baeba30f6e9Test
https://pubmed.ncbi.nlm.nih.gov/26975753Test
حقوق: CLOSED
رقم الانضمام: edsair.doi.dedup.....e7c9d737a2a640a9b5337baeba30f6e9
قاعدة البيانات: OpenAIRE