التفاصيل البيبلوغرافية
العنوان:
Development of a rapid method to isolate polyhydroxyalkanoates from bacteria for screening studies
المؤلفون:
Gary A. Leeke , Annabel V.L. Fitzgerald , Catherine A. Kelly , Mike J. Jenkins , Isaac Vizcaino-Caston , Tim W. Overton
المصدر:
Journal of Bioscience and Bioengineering . 121:101-104
بيانات النشر:
Elsevier BV, 2016.
سنة النشر:
2016
مصطلحات موضوعية:
0301 basic medicine , Time Factors , Cupriavidus necator , Bioengineering , Applied Microbiology and Biotechnology , Bioplastic , Polyhydroxyalkanoates , 03 medical and health sciences , chemistry.chemical_compound , Differential scanning calorimetry , Spectroscopy, Fourier Transform Infrared , Organic chemistry , Dimethyl Sulfoxide , Fourier transform infrared spectroscopy , Chloroform , Chromatography , Calorimetry, Differential Scanning , biology , Extraction (chemistry) , biology.organism_classification , 030104 developmental biology , chemistry , Solvents , Bacteria , Biotechnology
الوصف:
We describe a novel method of Polyhydroxyalkanoate (PHA) extraction using dimethyl sulphoxide (DMSO) for use in screening studies. Compared to conventional chloroform extraction, the DMSO method was shown to release comparable quantities of PHA from Cupriavidus necator cells, with comparable properties as determined using Fourier transform infrared spectroscopy and differential scanning calorimetry.
تدمد:
1389-1723
الوصول الحر:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3b19b3cd103efbceb039dbf6c6126f1Test https://doi.org/10.1016/j.jbiosc.2015.04.021Test
حقوق:
OPEN
رقم الانضمام:
edsair.doi.dedup.....c3b19b3cd103efbceb039dbf6c6126f1
قاعدة البيانات:
OpenAIRE
