Involvement of Lipoxygenase-dependent Production of Fatty Acid Hydroperoxides in the Development of the Hypersensitive Cell Death induced by Cryptogein on Tobacco Leaves
| العنوان: | Involvement of Lipoxygenase-dependent Production of Fatty Acid Hydroperoxides in the Development of the Hypersensitive Cell Death induced by Cryptogein on Tobacco Leaves |
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| المؤلفون: | Philippe Etienne, Anja Knoll, Jean-Pierre Blein, Jean-Jacques Bessoule, Lydie Suty, Christian Triantaphylidès, Jean-Pierre Agnel, Jean-Luc Montillet, Christine Rusterucci, Béatrice Alonso, Christine Battesti |
| المساهمون: | Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux Ségalen [Bordeaux 2], Phytopharmacie et Biochimie des Iteractions Cellulaires (PBIC), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB) |
| المصدر: | Journal of Biological Chemistry Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 1999, 274 (51), pp.36446-36455. ⟨10.1074/jbc.274.51.36446⟩ Journal of Biological Chemistry 51 (274), 36446-36455. (1999) Journal of Biological Chemistry, 1999, 274 (51), pp.36446-36455. ⟨10.1074/jbc.274.51.36446⟩ |
| بيانات النشر: | Elsevier BV, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | feuille, 0106 biological sciences, Lipid Peroxides, Programmed cell death, Necrosis, hypersensibilité, réaction hs, Lipoxygenase, nicotiana tabacum, Biology, 01 natural sciences, Biochemistry, Fungal Proteins, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, cryptogénie, Tobacco, medicine, peroxyde, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Lipase, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Cell Death, acide gras, Algal Proteins, food and beverages, Cell Biology, Respiratory burst, tabac, Chloroplast, Plants, Toxic, chemistry, biology.protein, lipids (amino acids, peptides, and proteins), peroxydation, medicine.symptom, Signal Transduction, 010606 plant biology & botany, Polyunsaturated fatty acid |
| الوصف: | Lipid peroxidation was investigated in relation with the hypersensitive reaction in cryptogein-elicited tobacco leaves. A massive production of free polyunsaturated fatty acid (PUFA) hydroperoxides dependent on a 9-lipoxygenase (LOX) activity was characterized during the development of leaf necrosis. The process occurred after a lag phase of 12 h, was accompanied by the concomitant increase of 9-LOX activity, and preceded by a transient accumulation of LOX transcripts. Free radical-mediated lipid peroxidation represented 10% of the process. Inhibition and activation of the LOX pathway was shown to inhibit or to activate cell death, and evidence was provided that fatty acid hydroperoxides are able to mimic leaf necrotic symptoms. Within 24 h, about 50% of leaf PUFAs were consumed, chloroplast lipids being the major source of PUFAs. The results minimize the direct participation of active oxygen species from the oxidative burst in membrane lipid peroxidation. They suggest, furthermore, the involvement of lipase activity to provide the free PUFA substrates for LOX. The LOX-dependent peroxidative pathway, responsible for tissue necrosis, appears as being one of the features of hypersensitive programmed cell death. |
| وصف الملف: | application/pdf |
| تدمد: | 0021-9258 1083-351X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ded283851ee5d1e6cd06c2359eee494aTest https://doi.org/10.1074/jbc.274.51.36446Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ded283851ee5d1e6cd06c2359eee494a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 1083351X |
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