An essential role for the Plasmodium Nek-2 Nima-related protein kinase in the sexual development of malaria parasites

التفاصيل البيبلوغرافية
العنوان: An essential role for the Plasmodium Nek-2 Nima-related protein kinase in the sexual development of malaria parasites
المؤلفون: Reininger, Luc, Tewari, Rita, Fennell, Clare, Holland, Zoe, Goldring, Dean, Ranford-Cartwright, Lisa, Billker, Oliver, Doerig, Christian
المصدر: Journal of Biological Chemistry. 284(31):20858-20868
مصطلحات موضوعية: Amino Acid Sequence, Animals, Genetically Modified, Cell Cycle Proteins/*metabolism, Culicidae/parasitology, DNA Replication, Erythrocytes/parasitology, Gene Expression Profiling, Gene Targeting, Green Fluorescent Proteins/metabolism, Humans, Life Cycle Stages, Malaria, Falciparum/*enzymology/parasitology/transmission, Meiosis, Molecular Sequence Data, NIMA-Related Kinase 1, Parasites/enzymology/genetics/growth & development, Phenotype, Plasmodium berghei/cytology/*enzymology/growth & development, Plasmodium falciparum/*enzymology/genetics/growth & development, Protein-Serine-Threonine Kinases/chemistry/genetics/*metabolism, Protozoan Proteins/genetics/*metabolism, RNA, Messenger/genetics/metabolism, Recombinant Fusion Proteins/metabolism, Sequence Alignment, *Sexual Development
الوصف: The molecular control of cell division and development in malaria parasites is far from understood. We previously showed that a Plasmodium gametocyte-specific NIMA-related protein kinase, nek-4, is required for completion of meiosis in the ookinete, the motile form that develops from the zygote in the mosquito vector. Here, we show that another NIMA-related kinase, Pfnek-2, is also predominantly expressed in gametocytes, and that Pfnek-2 is an active enzyme displaying an in vitro substrate preference distinct from that of Pfnek-4. A functional nek-2 gene is required for transmission of both Plasmodium falciparum and the rodent malaria parasite Plasmodium berghei to the mosquito vector, which is explained by the observation that disruption of the nek-2 gene in P. berghei causes dysregulation of DNA replication during meiosis and blocks ookinete development. This has implications (i) in our understanding of sexual development of malaria parasites and (ii) in the context of control strategies aimed at interfering with malaria transmission.
وصف الملف: print
الوصول الحر: https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-165880Test
قاعدة البيانات: SwePub
الوصف
تدمد:00219258
1083351X
DOI:10.1074/jbc.M109.017988