Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels
| العنوان: | Functional Interaction between the Scaffold Protein Kidins220/ARMS and Neuronal Voltage-Gated Na+ Channels |
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| المؤلفون: | Thierry Nieus, Fabio Benfenati, Annyesha Satapathy, Enrico Ferrea, Joachim Scholz-Starke, Fabrizia Cesca |
| المساهمون: | Cesca, Fabrizia, Satapathy, Annyesha, Ferrea, Enrico, Nieus, Thierry, Benfenati, Fabio, Scholz Starke, Joachim |
| المصدر: | Journal of biological chemistry (Online) 290 (2015): 18045–18055. doi:10.1074/jbc.M115.654699 info:cnr-pdr/source/autori:Cesca, Fabrizia; Satapathy, Annyesha; Ferrea, Enrico; Nieus, Thierry; Benfenati, Fabio; Scholz-Starke, Joachim/titolo:Functional Interaction between the Scaffold Protein Kidins220%2FARMS and Neuronal Voltage-Gated Na+ Channels./doi:10.1074%2Fjbc.M115.654699/rivista:Journal of biological chemistry (Online)/anno:2015/pagina_da:18045/pagina_a:18055/intervallo_pagine:18045–18055/volume:290 |
| بيانات النشر: | Elsevier BV, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Scaffold protein, hippocampus, Cercopithecus aethiop, Voltage-Gated Sodium Channels, Gating, Inbred C57BL, Bioinformatics, Biochemistry, Mice, Neurobiology, HEK293 Cell, Chlorocebus aethiops, Ankyrin, Premovement neuronal activity, Membrane Protein, Evoked Potentials, Cells, Cultured, Neurons, chemistry.chemical_classification, Cultured, gating, neuron, patch clamp, scaffold protein, sodium channel, medicine.anatomical_structure, COS Cells, Evoked Potential, Human, Cells, Animals, Cercopithecus aethiops, Gene Deletion, HEK293 Cells, Hippocampus, Humans, Membrane Proteins, Nerve Net, Cell Biology, Molecular Biology, Biology, Voltage Gated Sodium Channels, COS Cell, medicine, Patch clamp, Voltage-gated ion channel, hippocampu, Animal, Sodium channel, Voltage-Gated Sodium Channel, Neuron, Mice, Inbred C57BL, nervous system, chemistry, Biophysics, Cell, gating, hippocampus, neuron, patch clamp, scaffold protein, sodium channel, Animals, COS Cells, Cells, Cultured, Cercopithecus aethiops, Evoked Potentials, Gene Deletion, HEK293 Cells, Hippocampus, Humans, Membrane Proteins, Mice, Mice, Inbred C57BL, Nerve Net, Neurons, Voltage Gated Sodium Channels, Biochemistry, Cell Biology, Molecular Biology |
| الوصف: | Kidins220 (kinase D-interacting substrate of 220 kDa)/ankyrin repeat-rich membrane spanning (ARMS) acts as a signaling platform at the plasma membrane and is implicated in a multitude of neuronal functions, including the control of neuronal activity. Here, we used the Kidins220(-/-) mouse model to study the effects of Kidins220 ablation on neuronal excitability. Multielectrode array recordings showed reduced evoked spiking activity in Kidins220(-/-) hippocampal networks, which was compatible with the increased excitability of GABAergic neurons determined by current-clamp recordings. Spike waveform analysis further indicated an increased sodium conductance in this neuronal subpopulation. Kidins220 association with brain voltage-gated sodium channels was shown by co-immunoprecipitation experiments and Na(+) current recordings in transfected HEK293 cells, which revealed dramatic alterations of kinetics and voltage dependence. Finally, an in silico interneuronal model incorporating the Kidins220-induced Na(+) current alterations reproduced the firing phenotype observed in Kidins220(-/-) neurons. These results identify Kidins220 as a novel modulator of Nav channel activity, broadening our understanding of the molecular mechanisms regulating network excitability. |
| وصف الملف: | STAMPA |
| تدمد: | 0021-9258 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a9b85c5e2a61b9fffd583501b9867f9Test https://doi.org/10.1074/jbc.m115.654699Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....5a9b85c5e2a61b9fffd583501b9867f9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 00219258 |
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