التفاصيل البيبلوغرافية
| العنوان: |
MTA1 Coregulation of Transglutaminase 2 Expression and Function during Inflammatory Response. |
| المؤلفون: |
Ghanta, Krishna Sumanth1, Pakala, Suresh B.1, Reddy, Sirigiri Divijendra Natha1, Da-Qiang Li1, Nair, Sujit S.1, Kumar, Rakesh1 bcmrxk@gwumc.edu |
| المصدر: |
Journal of Biological Chemistry. 3/4/2011, Vol. 286 Issue 9, p7132-7138. 7p. |
| مصطلحات موضوعية: |
*TUMOR antigens, *CHROMATIN, *TRANSGLUTAMINASES, *ENZYMES, *CELLS, *MACROPHAGES |
| مستخلص: |
Although both metastatic tumor antigen 1 (MTA1), a master chromatin modifier, and transglutaminase 2 (TG2), a multifunctional enzyme, are known to be activated during inflammation, it remains unknown whether these molecules regulate inflammatory response in a coordinated manner. Here we investigated the role of MTA1 in the regulation of TG2 expression in bacterial lipopolysaccharide (LPS)-stimulated mammalian cells. While studying the impact of MTA1 status on global gene expression, we unexpectedly discovered that MTA1 depletion impairs the basal as well as the LPS-induced expression of TG2 in multiple experimental systems. We found that TG2 is a chromatin target of MTA1 and of NF-κB signaling in LPS-stimulated cells. In addition, LPS-mediated stimulation of TG2 expression is accompanied by the enhanced recruitment of MTA1, p65RelA, and RNA polymerase II to the NF-κB consensus sites in the TG2 promoter. Interestingly, both the recruitment of p65 and TG2 expression are effectively blocked by a pharmacological inhibitor of the NF-κB pathway. These findings reveal an obligatory coregulatory role of MTA1 in the regulation of TG2 expression and of the MTA1-TG2 pathway, at least in part, in LPS modulation of the NF-κB signaling in stimulated macrophages. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
Academic Search Index |
Full Text Finder