Soluble cell-bound and extracellular cyclodextrin glycosyltransferases ofBacillus macerans show identical enzymological characteristics and antigenicity
| العنوان: | Soluble cell-bound and extracellular cyclodextrin glycosyltransferases ofBacillus macerans show identical enzymological characteristics and antigenicity |
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| المؤلفون: | Noémi Nógrády, Péter Boross, J. Fachet, Attila Szentirmai, István Pócsi, Éva Katona, Viktória Jeney, József Tözsér |
| المصدر: | Journal of Basic Microbiology. 36:335-340 |
| بيانات النشر: | Wiley, 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Antigenicity, Lysis, Cyclodextrin, General Medicine, Cyclodextrin glycosyltransferase, Biology, Applied Microbiology and Biotechnology, Cell membrane, Enzyme, medicine.anatomical_structure, chemistry, Biochemistry, Glycosyltransferase, Extracellular, medicine, biology.protein |
| الوصف: | Soluble cell-bound and extracellular cyclodextrin glycosyltransferases of Bacillus macerans were purified to homogeneity using the subsequent steps of adsorption on starch, ion-exchange chromatography and gel filtration. The elution profiles and the relative molecular masses of the enzymes as well as their N-terminal sequences were found to be identical. Moreover, mouse monoclonal antibodies raised against the soluble cell-bound enzyme recognised equally well both the cell-bound and the extracellular enzyme preparations. Regarding that no signal peptide could be detected in either the cell-bound or the extracellular cyclodextrin glycosyltransferase the enzyme was probably entrapped between the cell membrane and the cell wall in the early stationary phase and was released during cell lysis without any modification. |
| تدمد: | 1521-4028 0233-111X |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::43c92d7881a6f0ca13546e54d6eca640Test https://doi.org/10.1002/jobm.3620360507Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........43c92d7881a6f0ca13546e54d6eca640 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15214028 0233111X |
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