التفاصيل البيبلوغرافية
| العنوان: |
Comparison of inhibitory efficacy of short interfering RNAs targeting different genes on Measles virus replication |
| المؤلفون: |
Jian Wang, Enjie Luo, Junyan Shi, Si Wang, Meilian Wang |
| المصدر: |
Journal of basic microbiology. 52(3) |
| سنة النشر: |
2011 |
| مصطلحات موضوعية: |
Small interfering RNA, Expression vector, Time Factors, biology, Genes, Viral, General Medicine, Transfection, biology.organism_classification, Virus Replication, Applied Microbiology and Biotechnology, Virology, Antiviral Agents, Nucleoprotein, Small hairpin RNA, Measles virus, RNA interference, Chlorocebus aethiops, Animals, Gene Silencing, RNA, Small Interfering, Gene, Vero Cells |
| الوصف: |
RNA interference (RNAi)-based short interfering RNAs (siRNAs) targeting the viral genes and the host cellular genes have been used to suppress Measles virus (MV) replication in vitro. In order to select suitable target genes and highly effective target sites for developing effective RNAi-mediated anti-MV therapy, in this study, nine short hairpin RNA (shRNA) expression vectors, which expressed siRNAs targeting the host celluar Rab9 GTPase gene, the viral large protein (L) gene and nucleoprotein (N) gene, respectively, were constructed and used to compare their ability to inhibit MV replication in Vero-E6 cells. The results showed that nine siRNAs targeting different genes exhibited different inhibitory efficacy on MV replication in vitro (about 23–94%), which could last at least 168 h post-infection. Of the nine siRNAs, R2, L1 and N2 more effectively decreased MV replication by over 90%. Furthermore, inhibitory efficacy on MV replication were increased and reached almost 100% when cells were transfected with pR2, pL1 and pN2 together. These results emphasis the importance of selecting suitable siRNA target sites for developing siRNAs-based drug therapy for MV, and demonstrate the potential of combination of siRNAs targeting different genes as a therapeutic approach to treat MV infection. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| تدمد: |
1521-4028 |
| الوصول الحر: |
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86b5c49ae8f584f917d1d0a102375d5cTest
https://pubmed.ncbi.nlm.nih.gov/22052457Test |
| حقوق: |
CLOSED |
| رقم الانضمام: |
edsair.doi.dedup.....86b5c49ae8f584f917d1d0a102375d5c |
| قاعدة البيانات: |
OpenAIRE |
