Ferric-pyoverdine recognition by Fpv outer membrane proteins of Pseudomonas protegens Pf-5
| العنوان: | Ferric-pyoverdine recognition by Fpv outer membrane proteins of Pseudomonas protegens Pf-5 |
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| المؤلفون: | Edward W. Davis, Sierra L. Hartney, Harald Gross, Joyce E. Loper, Sylvie Mazurier, Philippe Lemanceau, Maëva K. Girard, Samina Mehnaz |
| المساهمون: | Dept Bot & Plant Pathol, Oregon State University (OSU), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Rheinische Friedrich-Wilhelms-Universität Bonn, Forman Christian Coll Univ, USDA-ARS : Agricultural Research Service, USDA National Institute of Food and Agriculture [2006-35319-17427, 2008-35600-18770], Alexander von Humboldt Foundation (Georg Forster Fellowship) |
| المصدر: | Journal of Bacteriology Journal of Bacteriology, American Society for Microbiology, 2013, 195 (4), pp.765-776. ⟨10.1128/JB.01639-12⟩ |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Models, Molecular, Siderophore, Protein Conformation, [SDV]Life Sciences [q-bio], Iron, Mutagenesis (molecular biology technique), Sequence alignment, Microbiology, Mass Spectrometry, FERRIPYOVERDINE RECEPTOR, Substrate Specificity, NUCLEOTIDE-SEQUENCE ANALYSIS, 03 medical and health sciences, chemistry.chemical_compound, Pseudomonas protegens, HETEROLOGOUS SIDEROPHORES, Protein structure, SWISS-MODEL WORKSPACE, BACTERIAL SIDEROPHORES, Pseudomonas, FLUORESCENT PSEUDOMONAS, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, PUTIDA WCS358, Molecular Biology, Phylogeny, 030304 developmental biology, 0303 health sciences, Pyoverdine, biology, 030306 microbiology, Computational Biology, Gene Expression Regulation, Bacterial, Articles, biology.organism_classification, NONRIBOSOMAL PEPTIDE SYNTHETASES, chemistry, Biochemistry, Mutagenesis, [SDE]Environmental Sciences, Biological Assay, PSEUDOBACTIN RECEPTOR, NONFLUORESCENT PSEUDOMONAS, Bacterial outer membrane, Oligopeptides, Sequence Alignment, Gene Deletion, Bacterial Outer Membrane Proteins |
| الوصف: | International audience; The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric complexes of some pyoverdines produced by other strains of Pseudomonas spp. as sources of iron. Previously, phylogenetic analysis of the 45 TonB-dependent outer membrane proteins in Pf-5 indicated that six are in a well-supported clade with ferric-pyoverdine receptors (Fpvs) from other Pseudomonas spp. We used a combination of phylogenetics, bioinformatics, mutagenesis, pyoverdine structural determinations, and cross-feeding bioassays to assign specific ferric-pyoverdine substrates to each of the six Fpvs of Pf-5. We identified at least one ferric-pyoverdine that was taken up by each of the six Fpvs of Pf-5. Functional redundancy of the Pf-5 Fpvs was also apparent, with some ferric-pyoverdines taken up by all mutants with a single Fpv deletion but not by a mutant having deletions in two of the Fpv-encoding genes. Finally, we demonstrated that phylogenetically related Fpvs take up ferric complexes of structurally related pyoverdines, thereby establishing structure-function relationships that can be employed in the future to predict the pyoverdine substrates of Fpvs in other Pseudomonas spp. |
| تدمد: | 1098-5530 0021-9193 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56aa142ad6213267a8878f684ad25c00Test https://pubmed.ncbi.nlm.nih.gov/23222724Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56aa142ad6213267a8878f684ad25c00 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10985530 00219193 |
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