دورية
Genetic and Molecular Characteristics of X-Ray-Sensitive Mutants of Escherichia coliDefective in Repair Synthesis
| العنوان: | Genetic and Molecular Characteristics of X-Ray-Sensitive Mutants of Escherichia coliDefective in Repair Synthesis |
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| المؤلفون: | Kato, Takesi, Kondo, Sohei |
| المصدر: | Journal of Bacteriology; November 1970, Vol. 104 Issue: 2 p871-881, 11p |
| مستخلص: | Alleles responsible for X-ray-sensitive characteristics of three mutants of Escherichia coliB, which were also sensitive to ultraviolet (UV) irradiation, were mapped near metElocus, and named res-1, res-2, and res-3. All the res−mutants showed no host cell reactivability (Hcr−) for transducing deoxyribonucleic acid (DNA) of P1 phage irradiated by UV but they were Hcr+for infective DNA of P1 phage. Furthermore, they showed no detectable activity of DNA polymerase. Characteristics of allele res-1were studied in detail. The mutant res-1 uvr+showed an extensive degradation of DNA after UV irradiation. Double mutants carrying res-1 uvrA−, res-1 uvrB−, and res-1 uvrC−showed no marked increase in UV sensitivity beyond that of the uvr−single mutants and only negligible UV-induced DNA degradation. The uvr−mutations showed no such suppressive effect on DNA degradation induced by X rays in these double mutants. It is concluded that res−mutants are defective in the second step (repair synthesis) of the excision repair process and that DNA polymerase is partly responsible for the assumed resynthesis step. |
| قاعدة البيانات: | Supplemental Index |
Full Text Finder | تدمد: | 00219193 10985530 |
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| DOI: | 10.1128/jb.104.2.871-881.1970 |