دورية
Hydroxamate-mediated transport of iron controlled by ColV plasmids
العنوان: | Hydroxamate-mediated transport of iron controlled by ColV plasmids |
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المؤلفون: | Stuart, S J, Greenwood, K T, Luke, R K |
المصدر: | Journal of Bacteriology; July 1980, Vol. 143 Issue: 1 p35-42, 8p |
مستخلص: | A new high-affinity system for iron transport, associated with the presence of ColV plasmids, has been detected in Escherichia coli and partially characterized. The presence of such "iron-transport plasmids" in E. coli cells that are defective in enterochelin-mediated transport of iron enabled them to grow in media to which 2,2'-dipyridyl had been added to reduce availability of iron. In addition, the presence of plasmid deoxyribonucleic acid in a mutant defective in enterochelin biosynthesis was associated with a marked increase in the rate of radioactive-iron uptake. Plasmid-determined uptake of iron was distinct from previously recognized systems for iron transport in E. coli K-12, and the colicin V molecule appeared not to be directly involved. Hydroxylamine-nitrogen could be detected in cell pellets of ColV+ cultures, and similar material was detected in supernatant fluids of late log- or stationary-phase cultures. The hydroxamate material was not detected in cell pellets or culture supernatants of strains from which plasmids had been eliminated, and a 95% decrease in hydroxamate synthesis was observed when cells were grown in minimal medium containing 2 microM iron. |
قاعدة البيانات: | Supplemental Index |
تدمد: | 00219193 10985530 |
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DOI: | 10.1128/jb.143.1.35-42.1980 |