التفاصيل البيبلوغرافية
| العنوان: |
A leak-free head-out plethysmography system to accurately assess lung function in mice. |
| المؤلفون: |
Bruggink, Stephanie, Kentch, Kyle, Kronenfeld, Jason, Renquist, Benjamin J. |
| المصدر: |
Journal of Applied Physiology; Jul2022, Vol. 133 Issue 1, p104-118, 15p |
| مصطلحات موضوعية: |
PLETHYSMOGRAPHY, HOUSE dust mites, LUNG volume, LUNGS, MICE |
| مستخلص: |
Mice are a valuable model for elegant studies of complex, system-dependent diseases, including pulmonary diseases. Current tools to assess lung function in mice are either terminal or lack accuracy. We set out to develop a low-cost, accurate, headout variable-pressure plethysmography system to allow for repeated, nonterminal measurements of lung function in mice. Current head-out plethysmography systems are limited by air leaks that prevent accurate measures of volume and flow. We designed an inflatable cuff that encompasses the mouse’s neck preventing air leak. We wrote corresponding software to collect and analyze the data, remove movement artifacts, and automatically calibrate each dataset. This software calculates volume, inspiratory/expiratory time, breaths per minute, mid-expiratory flow, and end-inspiratory pause. To validate the use, we established that our plethysmography system accurately measured tidal breathing, the bronchoconstrictive response to methacholine, sex- and age-associated changes in breathing, and breathing changes associated with house dust mite sensitization. Our estimates of volume, flow, and timing of breaths are in line with published estimates, we observed dose-dependent decreases in volume and flow in response to methacholine (P < 0.05), increased lung volume, and decreased breathing rate with aging (P < 0.05), and that house dust mite sensitization decreased volume and flow (P < 0.05) while exacerbating the methacholine-induced increase in inspiratory time (P < 0.05). We describe an accurate, sensitive, low-cost, head-out plethysmography system that allows for longitudinal studies of pulmonary disease in mice. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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