Comparative transcriptome analysis of wild type and an oleaginous mutant strain of Desmodesmus sp. reveals a unique reprogramming of lipid metabolism under high light
| العنوان: | Comparative transcriptome analysis of wild type and an oleaginous mutant strain of Desmodesmus sp. reveals a unique reprogramming of lipid metabolism under high light |
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| المؤلفون: | Weijie Du, Yi Zhang, Changhai Wang, Tong Wang, Wu Chen, Hong Song, Meilin He |
| المصدر: | Journal of Applied Phycology. 31:2895-2910 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, biology, Chemistry, 010604 marine biology & hydrobiology, Mutant, Wild type, Desmodesmus, Lipid metabolism, Plant Science, Aquatic Science, biology.organism_classification, 01 natural sciences, Chloroplast, chemistry.chemical_compound, Biosynthesis, Biochemistry, Lipid biosynthesis, Fatty acid elongation, 010606 plant biology & botany |
| الوصف: | A mutant generated via ethylmethane sulfonate mutagenesis, Desmodesmus sp. G3, exhibited greater biomass and neutral lipid production over the wild type (WT) strain Desmodesmus sp. G41 in our previous study (Zhang et al., Bioresour Technol 207:268–275, 2016). G3 possessed a higher growth rate and lipid production than WT, with a biomass yield and total lipid content of 1302.86 mg L−1 and 48.58% respectively, which was promoted by 20.50% and 18.84%, compared to WT. Comparative transcriptome analysis was performed to elucidate the mechanism supporting enhanced biomass and lipid production in G3. A total of 1488 differentially expressed genes (DEGs) was identified comparing G3 and WT sequencing datasets, of which 753 and 735 genes were upregulated and downregulated respectively in G3. Pathway enrichment analysis indicated that ‘photosynthesis,’ ‘starch and sucrose metabolism,’ ‘fatty acid elongation,’ and ‘pyruvate metabolism’ were the notable represented DEGs-enriched pathways that might affect cell growth and lipid metabolism. Light harvesting capture was enhanced in G3. To protect PSII from photodamage, light harvesting complex-dependent non-photochemical quenching and state transitions was employed as the photoprotective strategy. De novo fatty acids (FAs) synthesis in chloroplast was downregulated in G3 while mitochondrion localized FAs elongation was enhanced to recycle carbon skeletons for lipid biosynthesis. Pyruvate mechanism was activated in G3 to generate acetyl-CoA through a pyruvate dehydrogenase bypass pathway, as the predominant pathway to provide precursor for lipids biosynthesis. |
| تدمد: | 1573-5176 0921-8971 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::883d2905c4e1e4806e4687124493c2daTest https://doi.org/10.1007/s10811-019-01821-wTest |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi...........883d2905c4e1e4806e4687124493c2da |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735176 09218971 |
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