التفاصيل البيبلوغرافية
| العنوان: |
Low- Z polymer sample supports for fixed-target serial femtosecond X-ray crystallography. |
| المؤلفون: |
Feld, Geoffrey K., Heymann, Michael, Benner, W. Henry, Pardini, Tommaso, Tsai, Ching-Ju, Boutet, Sébastien, Coleman, Matthew A., Hunter, Mark S., Li, Xiaodan, Messerschmidt, Marc, Opathalage, Achini, Pedrini, Bill, Williams, Garth J., Krantz, Bryan A., Fraden, Seth, Hau-Riege, Stefan, Evans, James E., Segelke, Brent W., Frank, Matthias |
| المصدر: |
Journal of Applied Crystallography; Aug2015, Vol. 48 Issue 4, p1072-1079, 8p |
| مصطلحات موضوعية: |
X-ray crystallography, FREE electron lasers, BIOMOLECULES, THIN films analysis, TRANSMISSION electron microscopy |
| مستخلص: |
X-ray free-electron lasers (XFELs) offer a new avenue to the structural probing of complex materials, including biomolecules. Delivery of precious sample to the XFEL beam is a key consideration, as the sample of interest must be serially replaced after each destructive pulse. The fixed-target approach to sample delivery involves depositing samples on a thin-film support and subsequent serial introduction via a translating stage. Some classes of biological materials, including two-dimensional protein crystals, must be introduced on fixed-target supports, as they require a flat surface to prevent sample wrinkling. A series of wafer and transmission electron microscopy (TEM)-style grid supports constructed of low- Z plastic have been custom-designed and produced. Aluminium TEM grid holders were engineered, capable of delivering up to 20 different conventional or plastic TEM grids using fixed-target stages available at the Linac Coherent Light Source (LCLS). As proof-of-principle, X-ray diffraction has been demonstrated from two-dimensional crystals of bacteriorhodopsin and three-dimensional crystals of anthrax toxin protective antigen mounted on these supports at the LCLS. The benefits and limitations of these low- Z fixed-target supports are discussed; it is the authors' belief that they represent a viable and efficient alternative to previously reported fixed-target supports for conducting diffraction studies with XFELs. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
