Prevalence of qnr genes among extended-spectrum β-lactamase-producing enterobacterial isolates in Barcelona, Spain
| العنوان: | Prevalence of qnr genes among extended-spectrum β-lactamase-producing enterobacterial isolates in Barcelona, Spain |
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| المؤلفون: | Montserrat Sabaté, Guillermo Prats, Juan José González-López, Alessandra Carattoli, Rosa Bartolomé, María Nieves Larrosa, S. Lavilla, Aurora García-Fernández |
| المصدر: | Journal of Antimicrobial Chemotherapy. 61:291-295 |
| بيانات النشر: | Oxford University Press (OUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | molecular cloning, chloramphenicol, kanamycin, antibiotic resistance, gene amplification, genetic organization, Klebsiella pneumoniae, polymerase chain reaction, medicine.disease_cause, bacterial protein, law.invention, Plasmid, law, amikacin, Prevalence, antibiotic agent, Pharmacology (medical), ceftazidime, protein qnrA, amoxicillin plus clavulanic acid, protein qnrB, hybridization, Polymerase chain reaction, Antibacterial agent, Genetics, biology, Escherichia coli Proteins, article, protein qnrS, genetic screening, Enterobacter, Enterobacteriaceae, unclassified drug, Infectious Diseases, bacterial gene, conjugation, aztreonam, bacterial DNA, cefepime, cefotaxime, ciprofloxacin, extended spectrum beta lactamase, gentamicin, nalidixic acid, quinoline derived antiinfective agent, sulfonamide, tetracycline, tobramycin, unclassified drug, antibiotic resistance, bacterium isolate, DNA sequence, Enterobacter cloacae, Escherichia coli, genetic transformation, isoelectric focusing, molecular probe, nonhuman, nucleotide sequence, plasmid, prevalence, Spain, Bacterial Proteins, beta-Lactamases, Humans, Spain, Microbiology (medical), Microbiology, Bacterial Proteins, medicine, Pharmacology, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification |
| الوصف: | Objectives To evaluate the presence of qnr genes among enterobacterial isolates carrying extended-spectrum beta-lactamases (ESBLs) in Barcelona, Spain. Methods Screening for the qnrA, qnrB and qnrS genes was carried out by PCR amplification with specific primers in 305 non-duplicate, clinically relevant ESBL-producing enterobacterial isolates obtained from February 2003 to August 2004. ESBLs from all qnr-positive isolates were characterized by isoelectric focusing, PCR amplification and DNA sequencing. Plasmid analysis was performed by S1 digestion and hybridization with specific probes for the qnr and bla genes. Plasmids containing qnr genes were transferred by conjugation or transformation. The genetic environment of qnrA1 in selected isolates was characterized by cloning experiments. Results Fifteen isolates, each from a different individual, carried qnr. Among them, 14 had qnrA1 (6 Klebsiella pneumoniae, 6 Enterobacter cloacae and 2 Escherichia coli isolates) and 1 had qnrS1 (K. pneumoniae). None of the isolates carried qnrB. Among the qnrA1-carrying isolates, 10 possessed both bla(CTX-M-9) and bla(SHV-12), 2 had both bla(CTX-M-9) and bla(SHV-92) and 2 had bla(CTX-M-9) alone. The isolate with qnrS1 possessed bla(SHV-12). The qnrA1 and ESBL genes were located together on plasmids ranging in size from 40 to 320 kb. qnrS1 and bla(SHV-12) were not located on the same plasmid. Transfer of quinolone resistance was successfully achieved from all but three isolates. The cloned region surrounding qnrA in two K. pneumoniae isolates revealed a novel genetic organization. Conclusions The prevalence of qnr among enterobacterial clinical isolates carrying ESBLs between 2003 and 2004 in Barcelona was 4.9%. qnrA1 was the most prevalent, whereas only one qnrS and no qnrB were detected. |
| تدمد: | 1460-2091 0305-7453 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::862b54e0eaac10af4bfcafc3cf8684aeTest https://doi.org/10.1093/jac/dkm448Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....862b54e0eaac10af4bfcafc3cf8684ae |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14602091 03057453 |
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