Oral administration of Moringa oleifera leaf powder relieves oxidative stress, modulates mucosal immune response and cecal microbiota after exposure to heat stress in New Zealand White rabbits
| العنوان: | Oral administration of Moringa oleifera leaf powder relieves oxidative stress, modulates mucosal immune response and cecal microbiota after exposure to heat stress in New Zealand White rabbits |
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| المؤلفون: | Talat Bilal Yasoob, Defu Yu, Xiaofeng Zhu, Heba M. Saad, Abdur Rauf Khalid, Zhen Zhang, Suqin Hang |
| المصدر: | Journal of Animal Science and Biotechnology Journal of Animal Science and Biotechnology, Vol 12, Iss 1, Pp 1-15 (2021) |
| بيانات النشر: | BioMed Central, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, Lipopolysaccharide, Veterinary medicine, Butyrate, Gut flora, medicine.disease_cause, Biochemistry, SF1-1100, Heat stress, Superoxide dismutase, chemistry.chemical_compound, Cecum, Internal medicine, Lactate dehydrogenase, SF600-1100, medicine, biology, Research, Short chain fatty acids, biology.organism_classification, Animal culture, Endocrinology, medicine.anatomical_structure, chemistry, Myeloperoxidase, Cecal microbiota, biology.protein, Animal Science and Zoology, Moringa oleifera leaf powder, Rabbits, Oxidative stress, Food Science, Biotechnology |
| الوصف: | BackgroundHeat stress (HS) disrupts the gut barrier allowing the uptake of lipopolysaccharide (LPS) and leads to an inflammatory response and changes in gut microbiota composition.Moringa oleiferaleaf powder (MOLP) has been proposed to combat HS, yet its alleviate role is currently under investigation. The current study investigated the effects of chronic HS and MOLP supplementation on changes in redox status and immune response of cecal mucosa along with alteration in cecal microbiota.MethodsA total of 21 young New Zealand White (NZW) rabbits (male) about 32 weeks old (mean body weight of 3318 ± 171 g) reared on a commercial pelleted diet were employed; divided into three groups (n = 7): control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS supplemented orally with MOLP (HSM, 35 °C) at 200 mg/kg body weight per day for 4 weeks.ResultsThe results demonstrated that MOLP supplementation increased organ index of cecal tissue compared with the HS group (P > 0.05). Levels of malonaldehyde (MDA) and activity of superoxide dismutase (SOD) as well as lactate dehydrogenase (LDH) were reduced in the cecal mucosa of the HSM group compared with the HS group. MOLP downregulated the contents of cecal mucosa LPS, several inflammatory markers (TNF-α/IL-1α/IL-1β), and myeloperoxidase (MPO) in the HSM group (P P IL-10,IFNG, andRLA, whereas both HS and MOLP increased the gene expression of fat digestion and absorption pathway, includingAPOA1,FABP1,FABP2,MTTP, andLOC100344166, compared to the CON group (P P Papillibacterwas higher in abundance in HSM groups compared to CON and HS groups (P P ConclusionIn conclusion, HS in growing rabbits resulted in alteration of cecal microbiota at phyla level as well as increased oxidative stress and expression of mucosal inflammatory genes. Whereas, oral MOLP supplementation elevated the relative weight of cecum, affected their immunological and cecal micro-ecosystem function by improving antioxidant status and down-regulating mucosal tissue inflammatory response. |
| اللغة: | English |
| تدمد: | 2049-1891 1674-9782 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba39112dc2230e28884aaa31d38e78caTest http://europepmc.org/articles/PMC8114525Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ba39112dc2230e28884aaa31d38e78ca |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20491891 16749782 |
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