التفاصيل البيبلوغرافية
| العنوان: |
Application of a novel biochip for rapid detection of mastitiscausing pathogens in bulk tank milk in Taiwan. |
| المؤلفون: |
Lee, K. H., Shy, Y. M., Lin, Y. T., Liu, L. Y., Lee, S. J., Chang, C. L., Wu, M. C., Chi, C. H. |
| المصدر: |
Journal of Animal Science; Aug2006 Supplement 1, Vol. 84, p92-92, 1/3p |
| مصطلحات موضوعية: |
BOVINE mastitis, MYCOPLASMA bovis, STREPTOCOCCUS agalactiae, BACTERIAL DNA, PATHOGENIC microorganisms, GENE amplification, DAIRY industry |
| مصطلحات جغرافية: |
TAIWAN |
| مستخلص: |
In order to efficiently prevent and treat bovine mastitis and minimize its impact on dairy industry in Taiwan, a sensitive, rapid, and specific test is required for identifying the mastitis-causing pathogens. In the present study, a biochip was developed in collaboration with DR. Chip Biotechnology Inc. in Taiwan. The biochip is capable of detecting 6 common species of mastitis-causing pathogens within 6 hours, including Streptococcus bovis, Streptococcus uberis, Streptococcus uberis, Streptococcus dysgalactiae, Escherichia coli and Staphylococcus aureus. The technique is based on DNA amplification of genes specific to the target pathogens and consists of 4 basic steps: DNA extraction of bacteria, PCR reaction, DNA hybridization, colorimetric reaction. The sensitivity and accuracy of this Biochip, in comparison with conventional culture method, were verified by analyzing serial diluted fresh whole milk that were separately inoculated with the 6 species of bacteria that mentioned previously. The Biochip had a lower detecting limit and showed results that have a similar trend to those from the conventional method. Thereafter, the Biochip was used for detecting bacteria in bulk tank milk samples from 207 DHI-participating dairy farms in 2004. The results show that only 6 (3%) farms were negative on all 6 species of pathogens monitored. Streptococcus bovis, detected in samples from 161 (79.7%) farms, was the most prevalent species, followed by Streptococcus uberis (n=123, 60.9%), Escherichia coli (n=92, 45.5%), Streptococcus dysgalactiae (n=62, 30.7%), Streptococcus agalactiae (n=55, 27.2%), and Staphylococcus aureus (n=19, 9.4%). Results from this study reveal that the biochip is a feasible tool for a rapid diagnose of mastitis-causing pathogens in milk, which might prevent and cure mastitis more effectively. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
