Fatty acid elongation and desaturation enzyme activities of bovine liver and subcutaneous adipose tissue microsomes

التفاصيل البيبلوغرافية
العنوان: Fatty acid elongation and desaturation enzyme activities of bovine liver and subcutaneous adipose tissue microsomes
المؤلفون: St. John, Lori C., Lunt, David K., Smith, Stephen B.
المصدر: Journal of Animal Science; March 1991, Vol. 69 Issue: 3 p1064-1073, 10p
مستخلص: Assay conditions were established for the fatty acid elongation and the A9desaturase enzyme systems of bovine liver and adipose tissue microsomes; rat liver microsomes were used as a reference. Overall fatty acid elongation was determined by measuring the incorporation of [2–14C]malonyl-coenzyme A (CoA) to 14C-labeled stearate. Rat liver elongation activity was .50 ± .02 nmolmin−1mg protein-1; bovine liver microsomal elongation activity was substantially lower (P < .05), with a mean value of .15 ± .02 nmolmin−1mg protein−1. The elongation activity of bovine s.c. adipose tissue microsomes (.42 ± .10 nmolmin−1mg protein−1) was not different (P > .05) from the activity observed in rat liver microsomes. To determine the fatty acid Δ9desaturase activity, microsomes were incubated in the presence of [l-14C]stearoyl-CoA and nicotinamide adenine dinucleotide (reduced form) (NADH), and the production of radioactively labeled oleate was quantified. Microsomal Δ9desaturase activity was similar in rat liver and bovine s.c. adipose tissue microsomes with rates of .15 ± .04 and .21 ± .05 nmol•min−1mg protein−1, respectively. However, no desaturase activity was detected in bovine liver microsomes, indicating that the liver is not a major site of oleate synthesis in this species. To investigate differences in fatty acid metabolism relative to breed type, eight Angus and seven Braford heifers were slaughtered at approximately 12 mo of age. Subcutaneous fat thickness over the 12th-13th thoracic vertebrae was greater in the Angus heifers than in the Braford heifers. However, no differences (P > .05) were observed in mean adipocyte size or number of cells per gram of adipose tissue between the Angus and Braford heifers. Similarly, there were no significant differences between the Angus and Braford s.c. adipose tissues for microsomal fatty acid elongation or Δ9desaturation, or for nicotinamide adenine dinucleotide phosphate (NADP)-malate dehydrogenase, fatty acid synthetase, or the pentose cycle reductases. The inability of bovine liver to convert stearate to oleate was in agreement with the fatty acid composition of the liver lipid, which had a smaller percentage of oleate and a higher percentage of stearate than s.c. adipose tissue.
قاعدة البيانات: Supplemental Index
الوصف
تدمد:00218812
15253163
DOI:10.2527/1991.6931064x