التفاصيل البيبلوغرافية
| العنوان: |
The effect of dietary protein levels on the expression of genes coding for four selected protein translation initiation factors in muscle tissue of Wujin pig. |
| المؤلفون: |
Liu, L. Y., Wang, J., Huang, Y., Pan, H. B., Zhang, X., Huang, Z. X., Zhao, S. M., Gao, S. Z. |
| المصدر: |
Journal of Animal Physiology & Animal Nutrition; Apr2014, Vol. 98 Issue 2, p310-317, 8p |
| مصطلحات موضوعية: |
LOW-protein diet, SWINE nutrition, GENE expression, TRANSLATION initiation factors (Biochemistry), BIOACCUMULATION, MUSCLE proteins, PROTEIN synthesis |
| مستخلص: |
The objective of this study was to investigate the regulatory mechanism underlying the increased muscle protein accumulation in pigs while were fed a high protein diet. The eukaryotic initiation factors (e IFs) have been reported to involve in muscle protein synthesis. We investigated the m RNA and protein expression levels of e IF2 B1, 4 A1, 4 B and 4 E in Wujin pigs fed either a high protein ( HP: 18%) or a low protein ( LP: 14%) diet at 30, 60 or 100 kg body weight, based on real-time PCR and western blotting analyses. Our results indicated that the expression levels of e IF2 B1 m RNA and protein were increased by HP diet at all body weight. The HP diet showed higher m RNA and protein levels of e IF4 B gene at 60 and 100 kg. The protein expression of e IF4 E phosphorylation was increased by HP diet only at 30 kg. These data suggested that the HP diet promoted porcine muscle protein accumulation mainly by up-regulating e IF2 B1, 4 B and 4 E rather than 4 A1 expression along the growth stages. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
