المؤلفون: Armelle Prunier, A. Hofer, E. Grindflek, Katia Feve, Denis Milan, Catherine Larzul, Annie Robic, Patrick Chevillon, Nathalie Iannuccelli, Juliette Riquet

المصدر: Journal of Animal Breeding and Genetics. 129:325-335

مصطلحات موضوعية: Regulation of gene expression, Genetics, endocrine system, 0303 health sciences, 0402 animal and dairy science, Androstenone, Promoter, 04 agricultural and veterinary sciences, General Medicine, Quantitative trait locus, Biology, 040201 dairy & animal science, Molecular biology, 3. Good health, 03 medical and health sciences, Exon, chemistry.chemical_compound, Food Animals, chemistry, SNP, Animal Science and Zoology, TEAD3, Gene, 030304 developmental biology

الوصف: A quantitative trait loci (QTL) for accumulation of androstenone in fat has been identified in an Large White × Meishan cross in a region of SSC7-containing TEAD3. In humans, TEAD3 is a transcription activator, known to be able to regulate the transcription of HSD3B. This enzyme is involved in the degradation of androstenone in the liver. In this study, porcine transcripts of TEAD3 were characterized and compared with mammalian transcripts. The complete structure of porcine TEAD3 gene was characterized including two 5' non-coding exons and one exon 5 not used in porcine transcripts. Variations were screened in sequences related to TEAD3: in exons, in flanking sequences of exons and in the promoter region. A SNP characterized at 726 bp at 5' of the first exon was tested on several pig populations without coherent and convincing results concerning its association with androstenone levels. We showed that in the liver of adult boars, the transcripts levels of TEAD3 and HSD3B were correlated. As in humans, it is possible that HSD3B is a target gene of TEAD3 in porcine liver. Nevertheless, no expression variation was observed for TEAD3 or HSD3B in liver between animals with different genotypes at the SNP. We concluded that this SNP was not the causal mutation of this QTL.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::217b1c1aa29ee56d8063c80bf52c2d98Test
https://doi.org/10.1111/j.1439-0388.2011.00979.xTest

المؤلفون: Sandrine Tacher, Florence Gondret, Julie Demars, Annie Robic, Jean Pierre Bidanel, Marie Pierre Sanchez, Juliette Riquet, Yvon Billon, Denis Milan

المصدر: Journal of Animal Breeding and Genetics. 129:316-324

مصطلحات موضوعية: 2. Zero hunger, Genetics, Chromosome 7 (human), 0303 health sciences, Candidate gene, 0402 animal and dairy science, 04 agricultural and veterinary sciences, General Medicine, Quantitative trait locus, Biology, 040201 dairy & animal science, 03 medical and health sciences, DLK1, Food Animals, Adipogenesis, CEBPA, Animal Science and Zoology, Allele, Gene, 030304 developmental biology

الوصف: A quantitative trait locus (QTL) affecting fatness in a way opposite to expectations based on breed means was mapped to swine chromosome 7 (SSC7) using crosses between Large White (LW) and Meishan (MS) founders. Defining the molecular fatness trait more explicitly would allow deducing positional candidate genes, for which expression differences must be analysed in experimental populations. First, mRNA levels of genes representing sequential steps in adipogenesis or involved in lipid metabolism were studied in backfat of pigs having homozygous LWQTL7/LWQTL7 or heterozygous LWQTL7/MSQTL7 alleles and considered at two ages. mRNA level of DLK1 expressed in preadipocytes was greater in MSQTL7/LWQTL7 pigs than in homozygous pigs at 28 days. Transcript abundances of CEBPA involved in differentiation, the prolipogenic FASN gene and the adipocyte-specific marker FABP4 were lower in MSQTL7/LWQTL7 pigs compared with LWQTL7/LWQTL7 pigs at 150 days. Because these results suggest a lag time in terminal differentiation associated with the MS allele, seven genes in the QTL interval were deduced as promising candidates for the QTL effect by bioinformatics analysis. Among them, PPARD and CDKN1A had lower expression levels in MSQTL7/LWQTL7 pigs at both ages. Genotype-related differences were observed in mRNA levels of PPARD target genes involved in cell differentiation (FZD7) or fatty acid oxidation (ACADL and ACOX1) at 150 days. These results re-evaluate the potential of PPARD to explain part of variation in pig adiposity.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::b40c5eb5f7b8f092381d6c6183461423Test
https://doi.org/10.1111/j.1439-0388.2011.00965.xTest