Remobilization causes site‐specific cyst formation in immobilization‐induced knee cartilage degeneration in an immobilized rat model
| العنوان: | Remobilization causes site‐specific cyst formation in immobilization‐induced knee cartilage degeneration in an immobilized rat model |
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| المؤلفون: | Tomoki Aoyama, Xiangkai Zhang, Shoki Yamaguchi, Momoko Nagai, Junichi Tajino, Hirotaka Iijima, Hiroshi Kuroki, Akira Ito |
| المصدر: | Journal of Anatomy |
| بيانات النشر: | John Wiley and Sons Inc., 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, musculoskeletal diseases, Cartilage, Articular, Male, Histology, degeneration, Cell Count, Degeneration (medical), Knee Joint, 03 medical and health sciences, Immobilization, 0302 clinical medicine, Chondrocytes, medicine, aggravation, Animals, Cyst, Femur, Tibia, Rats, Wistar, cartilage, Molecular Biology, Collagen Type II, Ecology, Evolution, Behavior and Systematics, 030203 arthritis & rheumatology, cyst, business.industry, Cysts, Cartilage, Cell Biology, Anatomy, Original Articles, medicine.disease, musculoskeletal system, Rats, remobilization, 030104 developmental biology, medicine.anatomical_structure, Hyaluronan Receptors, Cyst formation, Transitional Region, Original Article, business, Cartilage Diseases, Developmental Biology |
| الوصف: | An understanding of the articular cartilage degenerative process is necessary for the prevention and treatment of joint disease. The present study aimed to examine how long‐term immobilization‐induced cartilage degeneration is aggravated by remobilization. Sixty 8‐week‐old male Wistar rats were used in this study. The unilateral knee joint was immobilized using an external fixator for 8 weeks. The rats were killed at 0 and 3 days, and at 1, 2, 4 and 8 weeks after removing the fixator. After the rats were killed, the maximum knee extension angles were measured. Histological sections at the medial mid‐condylar region (non‐contact, transitional and contact regions of the femur and tibia) were prepared and scored. The cartilage thickness and number of chondrocytes were measured, and CD44 and Col2‐3/4c expression levels were assessed immunohistochemically. The histological assessment revealed progressive aggravation of cartilage degeneration in the transitional region, with a decreased number of chondrocytes and CD44‐positive chondrocytes as well as poor scoring over time, particularly in the tibia. Cyst formation was confirmed in the transitional region of the tibia at 8 weeks post‐remobilization. The cartilage thickness in the transitional region was thicker than that in the contact region, particularly in the tibia. Col2‐3/4c expression was observed in the non‐contact and transitional regions, and the knee extension angle was recovered. In conclusion, immobilization‐induced cartilage degeneration was aggravated by remobilization over time in the transitional region, followed by observations of a decreased number of chondrocytes and morphological disparity between different cartilage regions. |
| اللغة: | English |
| تدمد: | 1469-7580 0021-8782 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::81559a35ac0d4fac23e3bdbab4921a6eTest http://europepmc.org/articles/PMC5341580Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....81559a35ac0d4fac23e3bdbab4921a6e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14697580 00218782 |
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