Molecular Cloning and Characterization of a Novel Gene Encoding DREB Protein from Buchloe dactyloides (Nutt.) Engelm
| العنوان: | Molecular Cloning and Characterization of a Novel Gene Encoding DREB Protein from Buchloe dactyloides (Nutt.) Engelm |
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| المؤلفون: | Tiejun Zhang, Yan Li, Junmei Kang, Qingchuan Yang, Xiaoyan Hu, Yan Sun |
| المصدر: | Journal of Agricultural Science. 4 |
| بيانات النشر: | Canadian Center of Science and Education, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Genetics, Expressed sequence tag, Expression vector, Rapid amplification of cDNA ends, Complementary DNA, food and beverages, Coding region, Molecular cloning, Biology, Molecular biology, Peptide sequence, Gene |
| الوصف: | A 209bp expressed sequence tag (EST) was obtained with the degenerate primer technology, then based on the sequence of EST, the full-length cDNA of 1050 nucleotides was cloned from Buffalo grass by rapid amplification of cDNA ends (RACE). It was designated as BdDREB2, encoding a protein of 255 amino acids. The protein molecular weight was 28.39kDa, and the theoretical isoelectric point was 5.70. The BdDREB2 probably localized in nucleus and did not have signal peptide. A typical AP2/EREBP conserved domain was found in the coding region. The amino acid sequence compared by blast revealed high homology with DREB proteins of other plants, especially with the Buchloe-dactyloi DREB protein. The semi-quantitative analysis of expression of transcription showed: the expression of BdDREB2 gene reached the maximum after being treated by 20%PEG for 6h; BdDREB2 gene has the highest expression level in the root; the expression was significantly increased with the stress of drought (20% PEG) and high salt (3% NaCl), but not obvious with the stress of low temperature. Plant expression vector was constructed and transformed into the tobacco by the Agrobacterium-mediated methods. PCR amplification with the transgenic tobacco genome DNA indicated that the BdDREB2 gene had integrated in tobacco genome. RT-PCR showed that the BdDREB2 gene can be transcribed into mRNA in tobacco. |
| تدمد: | 1916-9760 1916-9752 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_________::7ada5c873460c9c6a5140ee0426d2c9cTest https://doi.org/10.5539/jas.v4n1p12Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi...........7ada5c873460c9c6a5140ee0426d2c9c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19169760 19169752 |
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