Fractionation of Grape Anthocyanin Classes Using Multilayer Coil Countercurrent Chromatography with Step Gradient Elution
| العنوان: | Fractionation of Grape Anthocyanin Classes Using Multilayer Coil Countercurrent Chromatography with Step Gradient Elution |
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| المؤلفون: | Stéphane Vidal, Véronique Cheynier, Emmanuelle Meudec, Yoji Hayasaka, George K. Skouroumounis |
| المساهمون: | Skouroumounis, George, Australian Wine Research Institute (AWRI), Cooperative Research Centre for Viticulture, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA) |
| المصدر: | Journal of Agricultural and Food Chemistry 4 (52), 713-719. (2004) Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 2004, 52 (4), pp.713-719. ⟨10.1021/jf034906a⟩ |
| بيانات النشر: | American Chemical Society (ACS), 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, grape, anthocyanins, liquid-liquid separation, multilayer coil countercurrent chromatography, step gradient elution, Chemical Fractionation, cyanin, 01 natural sciences, High-performance liquid chromatography, Mass Spectrometry, chromatographie hplc, chemistry.chemical_compound, Countercurrent chromatography, Vitis, Chromatography, High Pressure Liquid, anthocyane, Vegetal Biology, Chemistry, food and beverages, vin rose, 04 agricultural and veterinary sciences, monomer, 040401 food science, Agricultural sciences, oenologie, General Agricultural and Biological Sciences, hplc chromatography, rose wine, Fractionation, Mass spectrometry, monomère, 0404 agricultural biotechnology, Liquid–liquid extraction, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Countercurrent Distribution, Chromatography, Elution, fungi, 010401 analytical chemistry, Pomace, General Chemistry, 0104 chemical sciences, carbohydrates (lipids), Fruit, Anthocyanin, Spectrophotometry, Ultraviolet, Biologie végétale, Sciences agricoles |
| الوصف: | Grape anthocyanins from rose wine pomace and grape skins were fractionated by multilayer coil countercurrent chromatography (MLCCC). Tert-butyl methyl ether/n-butanol/acetonitrile/water acidified with trifluoroacetic acid (2/2/x/5) was chosen as the solvent system because of its demonstrated efficiency in separating anthocyanins. A method combining MLCCC and step gradient elution has been developed. This method enabled the fractionation of anthocyanins as series of glucosides, and the corresponding acetylated, coumaroylated, and caffeoylated derivatives. The different anthocyanins were identified on the basis of their UV spectra, their elution time on reversed-phase HPLC as well as by mass spectrometry. A polar-colored fraction free of anthocyanin monomers and corresponding to the material remaining in the stationary phase after the completion of the gradient was recovered. Keywords: Grape; anthocyanins; liquid−liquid separation; multilayer coil countercurrent chromatography; step gradient elution |
| وصف الملف: | application/pdf |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a871233e2cb0e87f7ed233d31c590157Test https://doi.org/10.1021/jf034906aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....a871233e2cb0e87f7ed233d31c590157 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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