المؤلفون: Yan-hong Shao, Lu Zhang, Jun Liu, Zong-cai Tu, Yao Zhang

المصدر: Journal of Agricultural and Food Chemistry. 69:14004-14012

مصطلحات موضوعية: biology, Chemistry, Immunogenicity, Milk allergy, Lactoglobulins, General Chemistry, Allergens, Immunoglobulin E, medicine.disease, biology.organism_classification, Enterobacteriaceae, Gastrointestinal Microbiome, Biochemistry, Glycation, RNA, Ribosomal, 16S, medicine, Humans, Ultrasonics, Milk Hypersensitivity, Bacteroides, General Agricultural and Biological Sciences, Digestion, Bacteroidaceae, Ruminococcaceae

الوصف: The effects of ultrasound combined with glycation (UCG) on the allergenicity and human microbial community of β-Lg during in vitro digestion were studied by ELISA, cell experiments, and 16S rRNA high-throughput sequencing. UCG modification and subsequent digestion significantly reduced allergenicity. The decrease in the allergenicity of β-Lg depended not only on the low digestibility of glycated β-Lg, which led to the decrease of some peptides with complete immunogenicity, but also the masking effect of glycation on allergen epitopes of β-Lg. Meanwhile, UCG modification and subsequent digestion could alter the structures of intestinal microbiota and the community abundance at phylum, family, and genus levels, such as Bacteroidota, Fusobacteriota, Enterobacteriaceae, Bacteroidaceae, Ruminococcaceae, Bacteroides, and Faecalibacterium. These results show that simulated in vitro digestion of modified β-Lg reduces allergenicity and alters human intestinal microbiota, which could provide a theoretical basis for studying the relationship between intestinal dysbiosis and cow's milk allergy.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682739dd0ca3139d2f2cc7590087c0a4Test
https://doi.org/10.1021/acs.jafc.1c03501Test

المؤلفون: Yujia Peng, Wei Dong, Peng Wan, Youlong Cao, Yamei Yan, Xiaoxiong Zeng, Kaiyin Huang

المصدر: Journal of Agricultural and Food Chemistry. 68:8863-8874

مصطلحات موضوعية: 0106 biological sciences, Rikenellaceae, biology, Chemistry, Firmicutes, 010401 analytical chemistry, Lachnospiraceae, Porphyromonadaceae, General Chemistry, Gut flora, biology.organism_classification, Ascorbic acid, 01 natural sciences, 0104 chemical sciences, Food science, General Agricultural and Biological Sciences, Peptococcaceae, 010606 plant biology & botany, Ruminococcaceae

الوصف: The modulating effect of 2-O-β-d-glucopyranosyl-l-ascorbic acid (AA-2βG), a natural derivative of ascorbic acid from the fruits of Lycium barbarum, on mice gut microbiota was investigated in the present study. It was found that AA-2βG was able to adjust the structure of mice gut microbiota, elevated the relative abundances of Verrucomicrobia, Porphyromonadaceae, Verrucomicrobiaceae, and Erysipelotrichaceae, and meanwhile reduced the relative abundances of Firmicutes, Lachnospiraceae, Rikenellaceae, Ruminococcaceae, Bdellovibrionaceae, Anaeroplasmataceae, and Peptococcaceae. Through the linear discriminant analysis effect size analysis, the key microbiota that were found to be significantly changed after long-term consumption of AA-2βG were Ruminococcaceae, Porphyromonadaceae, Lachnospiraceae, and Rikenellaceae. In addition, AA-2βG could upregulate pro-inflammatory cytokines, promote tight junctions between intestinal cells, facilitate the generation of short-chain fatty acids (SCFAs), and upregulate the mRNA expression level of SCFAs receptors, indicating that AA-2βG might promote organism health. The results demonstrated that AA-2βG might maintain organism health by modulating gut microbiota.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::415884280e5fcb85a98e83c2f80eca80Test
https://doi.org/10.1021/acs.jafc.0c04007Test

المؤلفون: Alexandra Ntemiri, Paul W. O'Toole, Eibhlís M. O'Connor, Catherine Stanton, R. Paul Ross, Céline Ribière

المساهمون: Department of Agriculture, Food and the Marine

المصدر: Journal of Agricultural and Food Chemistry. 67:2098-2112

مصطلحات موضوعية: Male, 0106 biological sciences, Aging, medicine.medical_treatment, Antibiotics, Lactose, Gut flora, 01 natural sciences, Feces, Mice, fluids and secretions, RNA, Ribosomal, 16S, Food science, Soy protein, "Humanized" mice, biology, Microbiota, Caseins, Biodiversity, "humanised" mice, soy prebiotic potential, Anti-Bacterial Agents, Milk, Models, Animal, Female, General Agricultural and Biological Sciences, Ruminococcaceae, medicine.drug_class, Frail Elderly, Health Promotion, medicine, Faecal microbiota, Animals, Humans, Aged, Prebiotic, Prebiotic potential, 010401 analytical chemistry, Lachnospiraceae, General Chemistry, biology.organism_classification, medicine.disease, Peptide Fragments, Diet, 0104 chemical sciences, Mice, Inbred C57BL, Lactose free milk, Prebiotics, Human nutrition, ageing, Cattle, Glycomacropeptide, Dysbiosis, 010606 plant biology & botany

الوصف: peer-reviewed Prebiotics may improve ageing-related dysbiosis. Milk is a source of nutrients including oligosaccharides whose prebiotic potential remains largely unexplored. We used a murine model to explore the effect of milk products on high diversity and lower diversity faecal microbiota from healthy and frail elderly subjects, respectively. Mice were treated with antibiotics and subsequently "humanised" with human faecal microbiota. The mice received lactose-free or whole milk, glycomacropeptide, or soy protein (control) supplemented diets for one month. The faecal microbiota was analysed by 16S rRNA gene amplicon sequencing. Lactose-free milk diet was as efficient as the control diet in retaining faecal microbiota diversity in mice. Both milk diets had a significant effect on the relative abundance of health-relevant taxa (e.g. Ruminococcaceae, Lachnospiraceae). The glycomacropeptide prebiotic activity previously observed in vitro was not replicated in vivo. However, these data indicate the novel prebiotic potential of bovine milk for human nutrition. ACCEPTED peer-reviewed

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efe55d6824b9722cd2b2ad51da9f7c27Test
https://doi.org/10.1021/acs.jafc.8b06414Test

المؤلفون: Yanan Wang, Jiacheng Yang, Shuai Wang, Lei Zhang, Desheng Qi, Kuntan Wu, Jiacai Zhang, Beiyu Zhang, Chong Li, Ao Yang

المصدر: Journal of agricultural and food chemistry. 67(17)

مصطلحات موضوعية: 0106 biological sciences, Male, Swine, Food Contamination, Anorexia, Weaning, Biology, Prevotellaceae, Gut flora, 01 natural sciences, Eating, Animal science, Glucagon-Like Peptide 1, medicine, Prevotella, Animals, 010401 analytical chemistry, Lachnospiraceae, General Chemistry, biology.organism_classification, Animal Feed, 0104 chemical sciences, Gastrointestinal Microbiome, Peptide YY, Growth Hormone, Female, medicine.symptom, General Agricultural and Biological Sciences, Trichothecenes, 010606 plant biology & botany, Hormone, Ruminococcaceae

الوصف: This study investigated the potential link between gut microbiota and deoxynivalenol (DON)-induced feed refusal. A total of 24 barrows were randomly divided into one of three diets containing 0.61 (control diet), 1.28, or 2.89 mg DON/kg feed for 28 days. Dietary exposure to DON at 2.89 mg/kg significantly decreased the relative abundances of unclassified_f_Lachnospiraceae, Phascolarctobacterium and Ruminococcaceae_UCG-014, whereas it increased Prevotella_9 and norank_f_Prevotellaceae in the cecal digesta. Moreover, the decreased relative abundance of unclassified_f_Lachnospiraceae induced by DON exposure was positively correlated with average daily feed intake. Exposure to DON increased the serum concentrations of glucagon-like peptide-1 and peptide YY but reduced the levels of serum growth hormone and insulin-like growth factor 1. In summary, these findings suggest that chronic dietary exposure to DON induces disturbances of intestinal microbiota. Disturbed appetite-regulating hormones and somatotropic-axis-hormone secretion induced by negative microbial changes could be the potential mechanisms for DON-induced anorexia.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c45b09ae32dc636b10297d9370e110aaTest
https://pubmed.ncbi.nlm.nih.gov/30977367Test