Mechanism of Reduction in Allergenicity and Altered Human Intestinal Microbiota of Digested β-Lactoglobulin Modified by Ultrasonic Pretreatment Combined with Glycation
| العنوان: | Mechanism of Reduction in Allergenicity and Altered Human Intestinal Microbiota of Digested β-Lactoglobulin Modified by Ultrasonic Pretreatment Combined with Glycation |
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| المؤلفون: | Yan-hong Shao, Lu Zhang, Jun Liu, Zong-cai Tu, Yao Zhang |
| المصدر: | Journal of Agricultural and Food Chemistry. 69:14004-14012 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, Chemistry, Immunogenicity, Milk allergy, Lactoglobulins, General Chemistry, Allergens, Immunoglobulin E, medicine.disease, biology.organism_classification, Enterobacteriaceae, Gastrointestinal Microbiome, Biochemistry, Glycation, RNA, Ribosomal, 16S, medicine, Humans, Ultrasonics, Milk Hypersensitivity, Bacteroides, General Agricultural and Biological Sciences, Digestion, Bacteroidaceae, Ruminococcaceae |
| الوصف: | The effects of ultrasound combined with glycation (UCG) on the allergenicity and human microbial community of β-Lg during in vitro digestion were studied by ELISA, cell experiments, and 16S rRNA high-throughput sequencing. UCG modification and subsequent digestion significantly reduced allergenicity. The decrease in the allergenicity of β-Lg depended not only on the low digestibility of glycated β-Lg, which led to the decrease of some peptides with complete immunogenicity, but also the masking effect of glycation on allergen epitopes of β-Lg. Meanwhile, UCG modification and subsequent digestion could alter the structures of intestinal microbiota and the community abundance at phylum, family, and genus levels, such as Bacteroidota, Fusobacteriota, Enterobacteriaceae, Bacteroidaceae, Ruminococcaceae, Bacteroides, and Faecalibacterium. These results show that simulated in vitro digestion of modified β-Lg reduces allergenicity and alters human intestinal microbiota, which could provide a theoretical basis for studying the relationship between intestinal dysbiosis and cow's milk allergy. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::682739dd0ca3139d2f2cc7590087c0a4Test https://doi.org/10.1021/acs.jafc.1c03501Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....682739dd0ca3139d2f2cc7590087c0a4 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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