An Extensive Description of the Peptidomic Repertoire of the Hen Egg Yolk Plasma
| العنوان: | An Extensive Description of the Peptidomic Repertoire of the Hen Egg Yolk Plasma |
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| المؤلفون: | Arena, Simona, Scaloni, Andrea |
| المصدر: | Journal of agricultural and food chemistry 66 (2018): 3239–3255. doi:10.1021/acs.jafc.8b01183 info:cnr-pdr/source/autori:Arena, Simona and Scaloni, Andrea/titolo:AN EXTENSIVE DESCRIPTION OF THE PEPTIDOMIC REPERTOIRE OF THE HEN EGG YOLK PLASMA/doi:10.1021%2Facs.jafc.8b01183/rivista:Journal of agricultural and food chemistry/anno:2018/pagina_da:3239/pagina_a:3255/intervallo_pagine:3239–3255/volume:66 |
| بيانات النشر: | American Chemical Society (ACS), 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Proteomics, 0301 basic medicine, food.ingredient, Antioxidant, Eggs, medicine.medical_treatment, Bioactive molecules, Fractionation, anorectic, anticancer, 01 natural sciences, Plasma, 03 medical and health sciences, food, Nutraceutical, Tandem Mass Spectrometry, Yolk, medicine, Animals, Yolk plasma, antibiofilm, Chemistry, 010401 analytical chemistry, General Chemistry, Egg Yolk, antiviral, 0104 chemical sciences, 030104 developmental biology, calcium-binding and anti-inflammatory activities, Biochemistry, Blood Vessels, antimicrobial, Female, Eggshell membrane, Peptides, General Agricultural and Biological Sciences, Chickens, Egg white |
| الوصف: | Hen egg is a raw material widely used for the preparation of food, pharmaceutical and cosmetoceutical products. Dedicated proteomic studies were accomplished on eggshell membrane, egg white, and yolk, identifying the most abundant proteins. No similar peptidomic studies have been performed so far. Only preliminary investigations on bioactive peptides in egg fractions and digestates were accomplished through functional screening assays, characterizing antioxidant, antibacterial, antiviral, immunomodulatory, and antihypertensive preparations and isolated components. This study fills this gap and provides a comprehensive picture of the peptides present in the yolk plasma of different hen egg types after 24 and 264 h of laying, taking advantage of a procedure based on a two-step fractionation followed by combined MALDI-TOF-TOF-MS- and nanoLC-ESI-Q-Orbitrap-MS/MS-based analysis. Six hundred and twenty-eight peptides were characterized as deriving from the proteolytic processing of larger protein components after the physiological action of chicken chymotrypsin-like and pepsin-like enzymes. Structural details on their post-translational modifications were also provided. Identified peptides were subjected to bioinformatic analysis and further compared with available data from the literature, ascertaining 198 peptides associable with putative antihypertensive, antimicrobial, anticancer, antiviral, antibiofilm, anorectic, calcium-binding, and anti-inflammatory activities. This analysis was often confirmative of previous experimental evidence on functional properties of unfractionated preparations or isolated molecules. These results further emphasize the bioactive action of yolk-derived peptides as related to egg consumption, and the potential use of these molecules as additive ingredients in the preparation of functional foods and cosmetics. |
| تدمد: | 1520-5118 0021-8561 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05f5f0c7c06e6fb549dffb998a041399Test https://doi.org/10.1021/acs.jafc.8b01183Test |
| حقوق: | RESTRICTED |
| رقم الانضمام: | edsair.doi.dedup.....05f5f0c7c06e6fb549dffb998a041399 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
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