التفاصيل البيبلوغرافية
| العنوان: |
Acacia catechu ethanolic bark extract induces apoptosis in human oral squamous carcinoma cells. |
| المؤلفون: |
Lakshmi, Thangavelu1, Ezhilarasan, Devaraj1,2 ezhild@gmail.com, Vijayaragavan, Rajagopal3, Bhullar, Sukhwinder Kaur4, Rajendran, Ramasamy5 |
| المصدر: |
Journal of Advanced Pharmaceutical Technology & Research. Oct-Dec2017, Vol. 8 Issue 4, p143-149. 7p. |
| مصطلحات موضوعية: |
*TREATMENT of oral cancer, *APOPTOSIS, THERAPEUTIC use of plant extracts |
| مستخلص: |
Oral cancer is in approximately 30% of all cancers in India. This study was conducted to evaluate the cytotoxic activity of ethanolic extract of Acacia catechu bark (ACB) against human squamous cell carcinoma cell line-25 (SCC-25). Cytotoxic effect of ACB extract was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide assay. A. catechu extract was treated SCC-25 cells with 25 and 50 µg/mL for 24 h. Apoptosis markers such as caspases-8 and 9, bcl-2, bax, and cytochrome c (Cyt-c) were done by RT-PCR. Morphological changes of ACB treated cells were evaluated using acridine orange/ethidium bromide (AO/EB) dual staining. Nuclear morphology and DNA fragmentation were evaluated using propidium iodide (PI) staining. Further, cell cycle analysis was performed using flow cytometry. A. catechu treatment caused cytotoxicity in SCC-25 cells with an IC50 of 52.09 µg/mL. Apoptotic marker gene expressions were significantly increased on ACB treatment. Staining with AO/EB and PI shows membrane blebbing and nuclear membrane distortion, respectively, and it confirms the apoptosis induction in SCC-25 cells. These results suggest that ACB extract can be used as a modulating agent in oral squamous cell carcinoma. [ABSTRACT FROM AUTHOR] |
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