Immunopathogenic CSF TCR repertoire signatures in virus-associated neurologic disease
| العنوان: | Immunopathogenic CSF TCR repertoire signatures in virus-associated neurologic disease |
|---|---|
| المؤلفون: | Nozuma, Satoshi, Enose-Akahata, Yoshimi, Johnson, Kory R., Monaco, Maria Chiara, Ngouth, Nyater, Elkahloun, Abdel, Ohayon, Joan, Zhu, Jun, Jacobson, Steven |
| المصدر: | JCI Insight JCI Insight, Vol 6, Iss 4 (2021) |
| بيانات النشر: | American Society for Clinical Investigation, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Infectious disease, Human T-lymphotropic virus 1, Receptors, Antigen, T-Cell, alpha-beta, Immunology, Receptors, Antigen, T-Cell, virus diseases, chemical and pharmacologic phenomena, hemic and immune systems, CD8-Positive T-Lymphocytes, HTLV-I Infections, Paraparesis, Tropical Spastic, Clone Cells, immune system diseases, Leukocytes, Mononuclear, Medicine, Humans, T cell receptor, Nervous System Diseases, Neurological disorders, Research Article |
| الوصف: | In this study, we examined and characterized disease-specific TCR signatures in cerebrospinal fluid (CSF) of patients with HTLV-1–associated myelopathy/tropical spastic paraparesis (HAM/TSP). TCR β libraries using unique molecular identifier–based methodologies were sequenced in paired peripheral blood mononuclear cells (PBMCs) and CSF cells from HAM/TSP patients and normal healthy donors (NDs). The sequence analysis demonstrated that TCR β repertoires in CSF of HAM/TSP patients were highly expanded and contained both TCR clonotypes shared with PBMCs and uniquely enriched within the CSF. In addition, we analyzed TCR β repertoires of highly expanded and potentially immunopathologic HTLV-1 Tax11-19–specific CD8+ T cells from PBMCs of HLA-A*0201+ HAM/TSP and identified a conserved motif (PGLAG) in the CDR3 region. Importantly, TCR β clonotypes of expanded clones in HTLV-1 Tax11-19–specific CD8+ T cells were also expanded and enriched in the CSF of the same patient. These results suggest that exploring TCR repertoires of CSF and antigen-specific T cells may provide a TCR repertoire signature in virus-associated neurologic disorders. |
| اللغة: | English |
| تدمد: | 2379-3708 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::07688102a18d45029894756e3614c212Test http://europepmc.org/articles/PMC7934934Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....07688102a18d45029894756e3614c212 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23793708 |
|---|