Antiviral Resistance of Biologic HIV-2 Clones Obtained From Individuals on Nucleoside Reverse Transcriptase Inhibitor Therapy
| العنوان: | Antiviral Resistance of Biologic HIV-2 Clones Obtained From Individuals on Nucleoside Reverse Transcriptase Inhibitor Therapy |
|---|---|
| المؤلفون: | Martin Schutten, Marchina E. van der Ende, Rob A. Gruters, Patrick H. M. Boers, Thoai Duong Ly, Christophe Guillon, Albert D. M. E. Osterhaus |
| المساهمون: | Erasmus MC other, Virology |
| المصدر: | Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, 25, 11-18. Lippincott Williams & Wilkins |
| بيانات النشر: | Ovid Technologies (Wolters Kluwer Health), 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | Adult, Male, Genes, Viral, Genotype, Molecular Sequence Data, HIV Infections, Biology, Virus, Nucleoside Reverse Transcriptase Inhibitor, Zidovudine, SDG 3 - Good Health and Well-being, immune system diseases, Consensus Sequence, medicine, Humans, Pharmacology (medical), Amino Acid Sequence, Didanosine, Reverse-transcriptase inhibitor, virus diseases, Lamivudine, Drug Resistance, Microbial, RNA-Directed DNA Polymerase, biochemical phenomena, metabolism, and nutrition, Nucleotidyltransferase, Virology, Reverse transcriptase, Phenotype, Infectious Diseases, HIV-2, Reverse Transcriptase Inhibitors, Drug Therapy, Combination, Female, medicine.drug |
| الوصف: | textabstractObjective: To study phenotypic and genotypic resistance of HIV-2 against nucleoside reverse transcriptase inhibitors (NRTI). Methods: Biologic HIV-2 clones were generated from 3 patients before and after initiation of antiretroviral therapy with zidovudine (AZT) in patient RH2-7, AZT and didanosine (ddI) in patient PH2-1, and after addition of lamivudine (3TC) to AZT monotherapy in patient RH2-5. The sensitivity to NRTI of the virus clones, as defined by the 50% inhibitory concentration (IC50), was determined in vitro. The predicted amino acid sequences of the reverse transcriptase proteins from these clones were determined. Results: Comparing the sensitivity of the biologic HIV-2 clones obtained after start of therapy with those from antiviral naive patients, resistance had developed to AZT (patients RH2-7 and RH2-5) and 3TC (patient PH2-1 and RH2-5). No resistance to AZT was observed in the biologic clone from PH2-1 obtained after start of therapy. The resistant clones from RH2-5 and PH2-1, but not RH2-7, contained amino acid mutations at positions where HIV-1 has been shown to mutate after AZT and 3TC treatment. Conclusions: Phenotypic resistance of HIV-2 to nucleoside analogues, which developed in HIV-2-infected patients treated with NRTIs, was associated with genotypic changes. Some of the mutations at amino acid positions in the HIV-2 reverse transcriptase gene corresponded with those involved in HIV-1 resistance, although no conventional mutations associated with resistance to AZT were observed. |
| تدمد: | 1525-4135 1077-9450 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f33d7bb6c4c5b88a7fb3e1fb6fe59dfTest https://doi.org/10.1097/00126334-200009010-00002Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....0f33d7bb6c4c5b88a7fb3e1fb6fe59df |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15254135 10779450 |
|---|