Cell cultures harbouring constructs of different pig promoter polymorphisms show different transcriptional efficiency in gene reporter systems

التفاصيل البيبلوغرافية
العنوان: Cell cultures harbouring constructs of different pig promoter polymorphisms show different transcriptional efficiency in gene reporter systems
المؤلفون: M. C. Savarese, Alessandro Nardone, Alessandra Crisà, Daniela Willems, Alessio Valentini, M. S. D’Andrea
المصدر: Italian Journal of Animal Science, Vol 6, Iss 1s (2010)
بيانات النشر: Taylor & Francis Group, 2010.
سنة النشر: 2010
مصطلحات موضوعية: Genetics, Candidate gene, Haplotype, Context (language use), Single-nucleotide polymorphism, Myostatin, Biology, genomic DNA, Gene expression, biology.protein, Animal Science and Zoology, lcsh:Animal culture, Gene, lcsh:SF1-1100
الوصف: Production traits variability among and within breeds, differences among developmental stages or the response to different environments are in part due to genetic factors that affect gene expression. Within the context of an Italian FIRB project, whose objective is to identify genes and molecular mechanisms affecting meat quality and production traits in pig, we studied the promoter regions of candidate genes selected on the basis of their physiological role in animal tissue development or composition. Genomic DNA was isolated from liver or muscle tissue of individuals belonging to Large White and Casertana breed. PCR primers were designed to amplify 5’ upstream region of SCD (Stearoyl-CoA Desaturase), LDLR (Low Density Lipoprotein Receptor), LEP (Leptin), MSTN (Myostatin), ACTA1 (Alpha-actin) and HFABP (Heart Fatty Acid Binding Protein) genes using sequences available at NCBI. A total of 19 single nucleotide polymorphisms (SNPs) not previously described were characterised. Some haplotypes, harbou...
اللغة: English
تدمد: 1594-4077
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4aa971ba7f6d7dbc307a5051a7f0a61fTest
http://www.aspajournal.it/index.php/ijas/article/view/1382Test
حقوق: OPEN
رقم الانضمام: edsair.doi.dedup.....4aa971ba7f6d7dbc307a5051a7f0a61f
قاعدة البيانات: OpenAIRE