التفاصيل البيبلوغرافية
| العنوان: |
Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme. |
| المؤلفون: |
Selenia, Miglietta, Familiari, Giuseppe, Guglielmelli, Alexa, Rosa, Paolo, Contardi, Marco, Prato, Mirko, Mangino, Giorgio, Petrozza, Vincenzo, Pani, Roberto, Calogero, Antonella, Athanassiou, Athanassia, Perotto, Giovanni, De Sio, Luciano |
| المصدر: |
Italian Journal of Anatomy & Embryology / Archivio Italiano di Anatomia Ed Embriologia; 2021 Supplement, Vol. 125, p71-71, 1p |
| مصطلحات موضوعية: |
GLIOBLASTOMA multiforme, GLIOMAS, GOLD nanoparticles, LIGHT scattering, LIGHT absorption, KERATIN, FOURIER transform infrared spectroscopy, CELL death |
| مستخلص: |
Biomedical applications of plasmonic nanoparticles (NPs), have steadily grown in the past 10 years. In particular, the ability of NPs to convert light into thermal energy (photothermal effect) has been exploited to develop a noninvasive, drug-free therapy defined as plasmonic photothermal (PPT). Indeed, when the NP surface, such as gold NPs (AuNPs), is illuminated, the strong absorption of light induces a significant temperature increase thereby achieving temperatures able to induce apoptosis in cancer cells [1]. Among the different proteins used to functionalize the surface of AuNPs, keratin (Ker) is still unexplored and no studies reporting its utilization in the field of PPT-based applications have been found. We tried to validate the exceptional biocompatibility of Ker-AuNPs and to perform PPT experiments on a human cell line (U87-MG) derived from glioblastoma, a brain tumor of glial origin, one of the most aggressive and currently untreatable cancers [2]. For comparison, cells were exposed to various concentrations of Ker-AuNPs (0-50 µM) for 24, 48 and 72 h. The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ?-potential, UV-Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy and thermography. In vitro experiments are performed using viability assays, fluorescence microscopy, cytometric analyses and PPT experiments. Morphological analysis of NPs, cellular uptake of Ker-AuNPs and ultrastructural changes were examined by transmission electron microscopy (TEM). Ultrastructural analysis shows the interactions between the U87-MG cells and the Ker-AuNPs; in particular free Ker-AuNPs appear close to the cell's apical surface, small clusters of Ker-AuNPs associated with the cell membrane invagination and well-packed Ker-AuNPs in the cytoplasm prevalently located in endosomes. Results have evidenced the extraordinary biocompatibility of Ker-AuNPs, even at the highest concentrations (50 µM) and after 72 h of in vitro incubation. Cytofluorimetric analysis evaluated both cellular uptake and PPT induced cell death; it turns out that 68% of U87-MG cells have been loaded with Ker-AuNPs, thus generating a reduction in their viability, after performing PPT experiments, of about 30%. The reported structural and functional properties pointed out these Ker-AuNPs as a promising biocompatible photothermal agent for PPT treatments against cancer-related diseases. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
