دورية أكاديمية
Comparison of the performance of Disk diffusion, Agar screening and E-test methods with Real-time PCR for the detection of methicillin resistant coagulase negative Staphylococcus strains isolated from clinical samples of Shahrekord university hospitals, 2008
| العنوان: | Comparison of the performance of Disk diffusion, Agar screening and E-test methods with Real-time PCR for the detection of methicillin resistant coagulase negative Staphylococcus strains isolated from clinical samples of Shahrekord university hospitals, 2008 |
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| المؤلفون: | Laleh Shariati, Majid Validi, Mana Shojapour, Ali Mohammad Hasheminia, Mohammad Amin Tabatabaiefar, Ali Karimi, Mohammad Reza Nafisi |
| المصدر: | Iranian South Medical Journal, Vol 15, Iss 2, Pp 93-100 (2012) |
| بيانات النشر: | Bushehr University of Medical Sciences, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Medicine (General) |
| مصطلحات موضوعية: | methicillin resistant coagulase negative staphylococcus, disk diffusion, agar screen, E-test, mecA gene, real- time PCR, Medicine (General), R5-920 |
| الوصف: | Background: Coagulase Negative staphylococci (CoNS) are nosocomial pathogens. The main objective of the study was to compare the performance of disk diffusion, E-test and agar screening methods with Real-time PCR technique for detection of methicillin resistance in coagulase negative staphylococci (MRCoNS), using Taqman® Real-time PCR for mecA as the “gold standard” comparison assay. Methods: 88 coagulase negative Staphylococcus isolates were identified out of 284 Staphylococcus isolates collected from Hajar and Kashani hospitals-shahrekod, Iran. Methicillin resistance strains were identified by several methods: Disk diffusion, Agar screening, E-test and Real-time PCR. The results of the tested methods were compared with those of the Real-time PCR by Chi square or Fisher’s exact tests. Results: Of the 88 coagulase negative Staphylococcus isolates tested, 46 isolates (52.3%) were mecA-positive and 42 isolates (47.7%) were mecA-negative. The results of all the tested methods had a statistically significant agreement with those of Real-time PCR. The E-test was 100% sensitive and specific for mecA presence. The sensitivity and specificity of oxacillin agar screen (6.0 μg/ml) method were 96% and 98%, respectively and the sensitivity and specificity of oxacillin Disk diffusion method were 91% and 90%, respectively. Conclusion: In the present study, E-test is proposed as the best phenotypic method. In case economic issue matters, the oxacillin agar screening method (6.0 μg/ml), which is suitable for the detection of MRCoNS due to its accuracy and low cost, is recommended. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Persian |
| تدمد: | 1735-4374 1735-6954 |
| العلاقة: | http://ismj.bpums.ac.ir/browse.php?a_code=A-10-3-257&slc_lang=en&sid=1Test; https://doaj.org/toc/1735-4374Test; https://doaj.org/toc/1735-6954Test |
| الوصول الحر: | https://doaj.org/article/7dbe6f6098884562a789bc8f848c1af8Test |
| رقم الانضمام: | edsdoj.7dbe6f6098884562a789bc8f848c1af8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17354374 17356954 |
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