دورية أكاديمية
Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for Neospora caninum and Toxoplasma gondii and Its Immune Response in an Animal Model
| العنوان: | Construction of an Adenovirus Vaccine Expressing the Cross-reactive Antigen AMA1 for Neospora caninum and Toxoplasma gondii and Its Immune Response in an Animal Model |
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| المؤلفون: | Lijun JIA, Huanping GUO, Mingming LIU, Yang GAO, Lei ZHANG, Hang LI, Suzhu XIE, Ningning ZHANG |
| المصدر: | Iranian Journal of Parasitology, Vol 13, Iss 2 (2018) |
| بيانات النشر: | Tehran University of Medical Sciences, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Neospora caninum, Toxoplasma gondii, AMA1 gene, Recombinant adenovirus, Immune response, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Background: We aimed to construct an adenovirus expressing a cross-reactive fragment of the apical membrane antigen 1 (AMA1) antigen and evaluated the concomitant immune response in BABL/c mice, allowing protection against N. caninum and T. gondii infection. Methods: The study was conducted in Agricultural College of Yanbian University, Yanji, Jilin, China In 2015-2016. Primers were designed using the AMA1 gene sequences of N. caninum (AB265823.1) and T. gondii (AF010264.1). After linearization of the plasmid ADV4-NcAMA1 and the framework plasmid pacAd5, a total of 293T cells were co-transfected and Ad5-NcAMA1 recombinant adenovirus were packed. BALB/c mice were inoculated. Simultaneously serum IgG antibody levels and IFN-γand IL-4 cytokine levels were determined by ELISA. After immunization three times in two weeks, each group of BABL/c mice were divided into two groups, respectively given intraperitoneal inoculation by the Neospora tachyzoite and Toxoplasma tachyzoite. Then we observed the clinical symptoms and statistical survival rate of mice. Results: The level of IgG in BABL/c mice immunized with Ad5-NcAMA1 was significantly increased when compared with that of pVAX1-NcAMA1 and PBS groups (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-7020 2008-238X |
| العلاقة: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2206Test; https://doaj.org/toc/1735-7020Test; https://doaj.org/toc/2008-238XTest |
| الوصول الحر: | https://doaj.org/article/307437d059b34cc5b3f49ac6fd6cd919Test |
| رقم الانضمام: | edsdoj.307437d059b34cc5b3f49ac6fd6cd919 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17357020 2008238X |
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