دورية أكاديمية
Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin
| العنوان: | Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin |
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| المؤلفون: | Yan Jiang, Linqiao Li, Qilu Pan, Xiaojing Du, Qian Han, Feixiang Ling, Rou Li, Lin Mai, Jianwei Huang, Shuyuan Chu, Libing Ma |
| المصدر: | Iranian Journal of Immunology, Vol 20, Iss 1, Pp 45-56 (2023) |
| بيانات النشر: | Shiraz University of Medical Sciences, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | cd4+t cell, il-17, mbd2, ovalbumin, th17 cell, Biology (General), QH301-705.5 |
| الوصف: | Background: Little is known about MBD2’s epigenetic regulation in the immune pathogenesis of CD4+T cell differentiation.Objective: This study attempted to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen ovalbumin (OVA).Methods: Mononuclear cells were separated from the spleen tissues of male C57BL/6 mice. The OVA interfered with the differentiation of splenic mononuclear cells and CD4+T cells. The CD4+T cells were obtained by magnetic beads and identified by CD4 labeled antibody. CD4+T cells were transfected with lentivirus to silence MBD2 gene. A methylation quantification kit was used to detect 5-mC levels.Results: The purity of CD4+T cells reached 95.99% after magnetic beads sorting. Treatment with 200 μg/mL OVA stimulated the CD4+T cells differentiation to Th17 cells and promoted the secretion of IL-17. After being induced, the Th17 cell ratio increased. 5-Aza inhibited the Th17 cell differentiation and the IL-17 level in a dose-dependent manner. Under the intervention of the Th17 induction and 5-Aza, MBD2 silencing inhibited the differentiation of Th17 cell, and decreased the IL-17 and 5-mC levels in the cell supernatants. MBD2 silencing reduced the scale of the Th17 cell and IL-17 levels in the OVA-treated CD4+T cells.Conclusion: MBD2 affected IL-17 and 5-mC levels by mediating the Th17 cell differentiation in splenic CD4+T cells that were interfered with 5-Aza. OVA induced Th17 differentiation and increased IL-17 levels, inhibited by MBD2 silencing. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1735-1383 1735-367X |
| العلاقة: | https://iji.sums.ac.ir/article_49135_36bb6e4f1b25026ffeeac8c8e9ef9ece.pdfTest; https://doaj.org/toc/1735-1383Test; https://doaj.org/toc/1735-367XTest |
| DOI: | 10.22034/iji.2023.93312.2212 |
| الوصول الحر: | https://doaj.org/article/2a80e9cc03b14b9eb0fbd22749f3a35fTest |
| رقم الانضمام: | edsdoj.2a80e9cc03b14b9eb0fbd22749f3a35f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17351383 1735367X |
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| DOI: | 10.22034/iji.2023.93312.2212 |