Time-Resolved Ultra-High Resolution Optical Coherence Tomography for Real-Time Monitoring of Selective Retina Therapy
| العنوان: | Time-Resolved Ultra-High Resolution Optical Coherence Tomography for Real-Time Monitoring of Selective Retina Therapy |
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| المؤلفون: | Sebastian Wolf, Jens Kowal, Andreas Ebneter, Patrick Steiner, Raphael Sznitman, Carsten Framme, Martin Zinkernagel, Boris Považay, Ralf Brinkmann, Christoph Meier, Lieselotte Berger |
| المصدر: | Investigative ophthalmology & visual science Investigative Ophthalmology & Visual Science INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Adult, Male, medicine.medical_specialty, Photodisruption, genetic structures, Retinal Pigment Epithelium, chemistry.chemical_compound, Retinal Diseases, Optical coherence tomography, medicine, Humans, Medical physics, Retina, Retinal pigment epithelium, medicine.diagnostic_test, business.industry, Fundus photography, Retinal, Fluorescein angiography, eye diseases, medicine.anatomical_structure, chemistry, Female, Laser Therapy, Tomography, sense organs, business, human activities, Tomography, Optical Coherence, Biomedical engineering |
| الوصف: | Purpose Selective retina therapy (SRT) has shown great promise compared to conventional retinal laser photocoagulation as it avoids collateral damage and selectively targets the retinal pigment epithelium (RPE). Its use however is challenging in terms of therapy monitoring and dosage because an immediate tissue reaction is not biomicroscopically discernibel. To overcome these limitations real time optical coherence tomography (OCT) might be useful to monitor retinal tissue during laser application. We have thus evaluated a proprietary OCT system for its capability of mapping optical changes introduced by SRT in retinal tissue. Methods Freshly enucleated porcine eyes covered in DMEM upon collection were utilized and a total of 175 scans from ex vivo porcine eyes were analyzed. The porcine eyes were used as an ex vivo model and results compared to two time resolved OCT scans recorded from a patient undergoing SRT treatment (SRT Vario Medical Laser Center Lübeck). In addition to OCT fluorescin angiography and fundus photography were performed on the patient and OCT scans were subsequently investigated for optical tissue changes linked to laser application. Results Biomicroscopically invisible SRT lesions were detectable in OCT by changes in the RPE / Bruch's complex both in vivo and the porcine ex vivo model. Laser application produced clearly visible optical effects such as hyper reflectivity and tissue distortion in the treated retina. Tissue effects were even discernible in time resolved OCT imaging when no hyper reflectivity persisted after treatment. Data from ex vivo porcine eyes showed similar to identical optical changes while effects visible in OCT appeared to correlate with applied pulse energy leading to an additional reflective layer when lesions became visible in indirect ophthalmoscopy. Conclusions Our results support the hypothesis that real time high resolution OCT may be a promising modality to obtain additional information about the extent of tissue damage caused by SRT treatment. Data shows that our ex vivo porcine model adequately reproduces the effects occurring in vivo and thus can be used to further investigate this promising imaging technique. |
| DOI: | 10.1167/iovs.15-17151 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08e50c1a836423c9283668b7804a957bTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....08e50c1a836423c9283668b7804a957b |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1167/iovs.15-17151 |
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