In Vitro Cytotoxic Evaluation of Processed Natural Coral in Human Osteoblasts
| العنوان: | In Vitro Cytotoxic Evaluation of Processed Natural Coral in Human Osteoblasts |
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| المؤلفون: | Thirumulu Ponnuraj Kannan, Suzina Sheikh Abdul Hamid, Siti Fadilah Abdullah, Abdul Rashid Ismail, Nor Shamsuria Omar, Abdul Rani Samsudin |
| المصدر: | International Journal of Toxicology. 30:443-451 |
| بيانات النشر: | SAGE Publications, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Neutral red, Cell, Tetrazolium Salts, Tetrazolium bromide, Apoptosis, Biocompatible Materials, Biology, Toxicology, Cell Line, Flow cytometry, chemistry.chemical_compound, Materials Testing, Cell Adhesion, medicine, Animals, Humans, Cytotoxic T cell, Cytotoxicity, Osteoblasts, medicine.diagnostic_test, Anthozoa, Flow Cytometry, Molecular biology, In vitro, Thiazoles, medicine.anatomical_structure, chemistry, Neutral Red, Immunology |
| الوصف: | This study aimed to evaluate the in vitro cytotoxic effects of locally produced processed natural coral (PNC) using human osteoblasts (HOS). Cytotoxicity was not observed when HOS cells were cultured with PNC, as assessed by (3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyl tetrazolium bromide; MTT) and Neutral Red (NR) assays at concentration up 200 mg/mL for up to 72 hours. Flow cytometry (FCM) analysis showed that PNC (200 mg/mL) did not decrease viability of HOS cells after 48 and 72 hours of treatment. In a cell attachment study, the HOS cells attached to the edge of the PNC disc, and later grew into the pores of the PNC disc. All results from these studies indicate that locally produced PNC material is noncytotoxic and favors the growth of HOS cells. |
| تدمد: | 1092-874X 1091-5818 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::949f96c35841b68ac944f0996730c42dTest https://doi.org/10.1177/1091581811399474Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....949f96c35841b68ac944f0996730c42d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1092874X 10915818 |
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