Could clinical photochemical internalisation be optimised to avoid neuronal toxicity?
| العنوان: | Could clinical photochemical internalisation be optimised to avoid neuronal toxicity? |
|---|---|
| المؤلفون: | O’Rourke, Caitriona, Hopper, Colin, MacRobert, Alexander J., Phillips, James B., Woodhams, Josephine H. |
| المصدر: | International Journal of Pharmaceutics |
| بيانات النشر: | Elsevier/North-Holland Biomedical Press, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Nervous system, 3D, Three-dimensional, Porphyrins, DRG, Dorsal root ganglion, Tetraphenylchlorin disulfonate (PubChem CID: 44177671), PCI, Photochemical Internalisation, Bleomycin (PubChem CID: 5360373), Article, TPPS2a, Meso-tetraphenylporphine, Bleomycin, Drug Delivery Systems, Cell Line, Tumor, Ganglia, Spinal, Neoplasms, Humans, PDT, Photodynamic therapy, ComputingMethodologies_COMPUTERGRAPHICS, Neurons, Photochemical Internalisation, 3D culture models, Photosensitizing Agents, CNS, Central nervous system, HNC, Head and neck cancer, TPCS2a, Tetraphenylchlorin disulfonate, Coculture Techniques, Photosensitisers, Photochemotherapy, Meso-tetraphenylporphine (PubChem CID: 70186), Neuroglia, ROS, Reactive oxygen species |
| الوصف: | Graphical abstract Photochemical Internalisation (PCI) is a novel drug delivery technology in which low dose photodynamic therapy (PDT) can selectively rupture endo/lysosomes by light activation of membrane-incorporated photosensitisers, facilitating intracellular drug release in the treatment of cancer. For PCI to be developed further, it is important to understand whether nerve damage is an impending side effect when treating cancers within or adjacent to nervous system tissue. Dorsal root ganglion (DRG) neurons and their associated satellite glia were subjected to PCI treatment in a 3D co-culture system following incubation with photosensitisers: meso-tetraphenylporphine (TPPS2a) or tetraphenylchlorin disulfonate (TPCS2a) and Bleomycin. Results from the use of 3D co-culture models demonstrate that a cancer cell line PCI30 and satellite glia were more sensitive to PCI than neurons and mixed glial cells, athough neurite length was affected. Neurons in culture survived PCI treatment under conditions sufficient to kill tumour cells, suggesting cancers within or adjacent to nervous system tissue could be treated with this novel technology. |
| اللغة: | English |
| تدمد: | 1873-3476 0378-5173 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid________::b29124123c7ec4815bf8370cb8a1f4d1Test http://europepmc.org/articles/PMC5571751Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid..........b29124123c7ec4815bf8370cb8a1f4d1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733476 03785173 |
|---|