التفاصيل البيبلوغرافية
| العنوان: |
Development and biological evaluation of pNIPAM-based nanogels as vaccine carriers. |
| المؤلفون: |
Soriano Pérez, Maria Laura1 (AUTHOR), Funes, Javier Alejandro1 (AUTHOR), Flores Bracamonte, Carolina2 (AUTHOR), Ibarra, Luis Exequiel3 (AUTHOR), Forrellad, Marina Andrea4 (AUTHOR), Taboga, Oscar4 (AUTHOR), Cariddi, Laura Noelia3 (AUTHOR), Salinas, Facundo José5 (AUTHOR), Ortega, Hugo Héctor5 (AUTHOR), Alustiza, Fabrisio1 (AUTHOR), Molina, Maria1,6 (AUTHOR) mamolina7@gmail.com |
| المصدر: |
International Journal of Pharmaceutics. Jan2023, Vol. 630, pN.PAG-N.PAG. 1p. |
| مصطلحات موضوعية: |
*NANOGELS, *ANTIBODY-dependent cell cytotoxicity, *HUMORAL immunity, *ACTINOBACILLUS pleuropneumoniae, *ALUMINUM hydroxide, *ANTIBODY titer |
| مستخلص: |
[Display omitted] • Thermoresponsive nanogeles are developed as vaccine platform. • Nanogels are internalized by macrophage cell line in absence of cytotoxicity. • Fluorescence of nanogels is detected in faeces after intranasal inoculation in mice. • OmlA-nanogels formulation produces detectable titer of OmlA-specific IgG antibodies. "Smart" nanogels are an attractive tool for the development of new strategies of immunization in veterinary medicine. Here, we reported the synthesis and physicochemical characterization of thermoresponsive nanogels based on poly(N -isopropylacrylamide) (p NIPAM) and their in vitro, ex vivo and in vivo (mice model) performance. Smart nanogels of ca. 250 nm, with a transition temperature of 32 °C were obtained by precipitation polymerization. Assays to evaluate p NIPAM nanogels cytotoxicity were performed in different cell lines showing high biocompatibility (>70 %). The efficient internalization of the system was studied by confocal microscopy as well as flow cytometry. The ability to protect and deliver antigens was analyzed using the outer membrane lipoprotein A (OmlA), an important virulence factor of Actinobacillus pleuropneumoniae (App) cause of porcine pleuropneumonia. This lipoprotein was synthesized by recombinant technology and its technique was also described. The biodistribution of p NIPAM nanogels administered intranasally was performed in vivo and ex vivo through Pearl Imaging System, which showed that nanogels were kept mostly in the lungs during the evaluated time. Besides, the efficacy of the proposal nanogel-based vaccine was studied in vivo by measuring the antibody titers of BALB/c mice inoculated with OmlA encapsulated into p NIPAM nanogels compared to OmlA plus aluminum hydroxide adjuvant. The results proved the ability of nanogels to stimulate a humoral immune response. Therefore, we have demonstrated that p NIPAM nanogels can be used as an efficient platform for vaccine nanocarriers. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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