Liposome mediated-CYP1A1 gene silencing nanomedicine prepared using lipid film-coated proliposomes as a potential treatment strategy of lung cancer
| العنوان: | Liposome mediated-CYP1A1 gene silencing nanomedicine prepared using lipid film-coated proliposomes as a potential treatment strategy of lung cancer |
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| المؤلفون: | Zhirong Zhang, Waqar Ahmed, Xun Sun, Mengtian Zhang, Ka-Wai Wan, Abdelbary Elhissi, Qin Wang, Mohamed A. Elrayess, David A. Phoenix |
| المصدر: | International journal of pharmaceutics. 566 |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Male, Lung Neoplasms, CYP1A1, Pharmaceutical Science, Mice, Nude, Apoptosis, 02 engineering and technology, Gene delivery, 030226 pharmacology & pharmacy, 03 medical and health sciences, 0302 clinical medicine, RNA interference, Tobacco, B200 Pharmacology, Toxicology and Pharmacy, medicine, polycyclic compounds, Cytochrome P-450 CYP1A1, Gene silencing, Animals, Humans, Cationic liposome, J510 Materials Technology, F200 Materials Science, Gene Silencing, RNA, Messenger, RNA, Small Interfering, Lung, Carcinogen, Cancer, Liposome, Mice, Inbred BALB C, Chemistry, Smoking, B230, respiratory system, 021001 nanoscience & nanotechnology, medicine.disease, Lipids, Nanomedicine, A549 Cells, siRNA, Liposomes, Cancer research, 0210 nano-technology, Methylcholanthrene |
| الوصف: | The occurrence of lung cancer is linked with tobacco smoking, mainly through the generation of polycyclic aromatic hydrocarbons (PAHs). Elevated activity of cytochrome P4501A1 (CYP1A1) plays an important role in the metabolic processing of PAHs and its carcinogenicity. The present work aimed to investigate the role of CYP1A1 gene in PAH-mediated growth and tumor development in vitro and using an in vivo animal model. RNAi strategy was utilized to inhibit the overexpression of CYP1A1 gene using cationic liposomes generated using a lipid film-coated proliposome microparticles. Treatment of PAH-induced human alveolar adenocarcinoma cell line with cationic liposomes carrying CYP1A1 siRNA resulted in down regulation of CYP1A1 mRNA, protein as well as its enzymatic activity, triggering apoptosis and inhibiting multicellular tumor spheroids formation in vitro. Furthermore, silencing of CYP1A1 gene in BALB/c nude xenografts inhibited tumor growth via down regulation of CYP1A1 expression. Altogether, our findings showed that liposome-based gene delivery technology is a viable and stable approach for targeting cancer causing genes such as CY1PA1. This technology facilitated by the use of sugar particles coated with lipid films has demonstrated ability to generate anticancer effects that might be used in the future for therapeutic intervention and treatment of lung cancer. [Abstract copyright: Copyright © 2019. Published by Elsevier B.V.] |
| وصف الملف: | application/pdf |
| تدمد: | 1873-3476 0378-5173 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c85ac56ffce84cd7d5e11b3ed65c010Test https://pubmed.ncbi.nlm.nih.gov/31051230Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4c85ac56ffce84cd7d5e11b3ed65c010 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18733476 03785173 |
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