Differential expression and functions of Ehm2 transcript variants in lung adenocarcinoma
| العنوان: | Differential expression and functions of Ehm2 transcript variants in lung adenocarcinoma |
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| المؤلفون: | Mu Hu, Wen Guo Jiang, Shenglan Li, Baolan Li, Shan Cheng, Hefen Yu, Xiuyi Zhi, Jian Ma, Yang Si |
| المصدر: | International Journal of Oncology. |
| بيانات النشر: | Spandidos Publications, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, 0301 basic medicine, Cancer Research, Epithelial-Mesenchymal Transition, Lung Neoplasms, Down-Regulation, Adenocarcinoma of Lung, Biology, 03 medical and health sciences, 0302 clinical medicine, Cell Movement, medicine, Humans, Lung cancer, A549 cell, Regulation of gene expression, Gene knockdown, Oncogene, Cancer, Middle Aged, medicine.disease, Survival Analysis, Gene Expression Regulation, Neoplastic, Alternative Splicing, Cytoskeletal Proteins, 030104 developmental biology, Oncology, A549 Cells, 030220 oncology & carcinogenesis, Cancer cell, Cancer research, Adenocarcinoma, Female |
| الوصف: | Ehm2 [also known as erythrocyte membrane protein band 4.1‑like protein 4B (EPB41L4B)] is a member of the NF2/ERM/4.1 superfamily. The overexpression of Ehm2 has been observed in metastatic cancer cells. Through alternative splicing, the Ehm2 gene produces two transcript variants that encode the two different isoforms, Ehm2/1 and Ehm2/2. The biological functions of these different Ehm2 transcript variants remain unclear. The present study aimed to determine the expression of the Ehm2 variants in lung adenocarcinoma and their involvement in the disease progression of the patients. The expression of Ehm2 transcript variants in human lung adenocarcinoma tissues was analyzed using immunohistochemistry and western blot analysis. Ehm2 variants were overexpressed or knocked down in A549 human lung adenocarcinoma cells. The consequent effects of the genetic modifications on the cellular functions of lung cancer cells were then examined using in vitro cell viability, invasion and migration assays. The expression of epithelial‑mesenchymal transition (EMT)‑related markers was evaluated by western blot analysis in the cell models. The association of Ehm2 variant expression with patient survival was analyzed using Kaplan‑Meier survival analysis. The expression of Ehm2/1 was significantly decreased in lung cancers compared with the paired normal lung tissues (P |
| وصف الملف: | application/pdf |
| تدمد: | 1791-2423 1019-6439 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d84ab6da5f3ac332ca7f361ebfedc5eTest https://doi.org/10.3892/ijo.2019.4732Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....7d84ab6da5f3ac332ca7f361ebfedc5e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17912423 10196439 |
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